BACKGROUND The aim of this study was to investigate the potential anti-obesity effects of Camellia nitidissima Chi flower extract (Cnfe) by examining its effects in terms of the regulation of lipid levels and modulation of gut microbiota in rats with high-fat-diet-induced obesity. RESULTS Our results demonstrated that Cnfe significantly decreased weight gain by reducing appetite and decreasing high-fat food intake. Further, Cnfe restored normal lipid metabolism, and improved insulin sensitivity and glucose tolerance in rats fed a high-fat diet. Real-time RT-PCR and western blot results showed that Cnfe significantly decreased the expression of genes and proteins involved in adipogenesis and lipogenesis, and up-regulated the expression of lipolysis genes. 16S rRNA sequencing of feces showed that Cnfe dramatically reversed dysbacteriosis in rats with high-fat-diet-induced obesity by decreasing the abundance of Firmicutes and increasing that of Bacteroidetes and Verrucomicrobia at the phylum level. CONCLUSION The results demonstrate that Cnfe is a potential anti-obesity prebiotic nutrient that can prevent weight gain, ameliorate obesity-related dyslipidemia and hyperglycemia, inhibit liver fat accumulation, and modulate gut microbiota. This article is protected by copyright. All rights reserved.

中文翻译：

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山茶花提取物减轻肥胖及相关并发症并调节高脂饮食诱导肥胖大鼠的肠道菌群组成

背景本研究的目的是通过研究山茶花提取物（Cnfe）在调节高脂饮食大鼠的脂质水平和肠道微生物群方面的作用来研究其潜在的抗肥胖作用。诱发肥胖。结果 我们的结果表明，Cnfe 通过降低食欲和减少高脂肪食物的摄入量显着降低了体重增加。此外，Cnfe 恢复了正常的脂质代谢，并改善了喂食高脂肪饮食的大鼠的胰岛素敏感性和葡萄糖耐量。实时 RT-PCR 和蛋白质印迹结果表明，Cnfe 显着降低了参与脂肪生成和脂肪生成的基因和蛋白质的表达，并上调了脂肪分解基因的表达。粪便的 16S rRNA 测序表明，Cnfe 通过在门水平上降低厚壁菌门的丰度并增加拟杆菌门和疣微菌门的丰度，显着逆转了高脂饮食诱导的肥胖大鼠的菌群失调。结论 结果表明，Cnfe 是一种潜在的抗肥胖益生元营养素，可以防止体重增加，改善肥胖相关的血脂异常和高血糖，抑制肝脏脂肪积累，调节肠道微生物群。本文受版权保护。版权所有。改善肥胖相关的血脂异常和高血糖，抑制肝脏脂肪堆积，调节肠道菌群。本文受版权保护。版权所有。改善肥胖相关的血脂异常和高血糖，抑制肝脏脂肪堆积，调节肠道菌群。本文受版权保护。版权所有。