Polygonatum odoratum (Mill.) Druce (P. odoratum) is a traditional Chinese herb. The isoflavonoids of P. odoratum are important medicinal bioactive compounds for the treatment of hypoimmunity, rheumatic heart disease, cardiovascular diseases, and diabetes. This study used RNA sequencing to identify potential genes regulating isoflavonoid biosynthesis in P. odoratum. To do this, we generated an overview of metabolic and secondary metabolic pathways, using MAPMAN, and identified 96 genes encoding seven key enzymes involved in isoflavonoid biosynthesis through their KEGG annotation. Alignment of phenylalanine ammonia-lyase or chalcone synthase amino acid sequences revealed well-conserved sequences, spatial structures, and active sites. We also verified the expression of key genes encoding phenylalanine ammonia-lyase and chalcone synthase by quantitative real-time PCR (qRT-PCR). This analysis of the isoflavonoid biosynthesis pathway and its crucial enzymes in P. odoratum lays the foundation for uncovering the regulatory mechanism of isoflavonoid biosynthesis.

中文翻译：

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玉竹黄连的转录组分析发现异黄酮类生物合成的推定基因。

玉竹（M .. Druce）（P. odoratum）是传统的中草药。香雪竹的异黄酮是重要的药用生物活性化合物，可用于治疗免疫力低下，风湿性心脏病，心血管疾病和糖尿病。这项研究使用RNA测序来确定潜在的基因，以调节香竹中的异黄酮生物合成。。为此，我们使用MAPMAN生成了代谢和次级代谢途径的概述，并通过其KEGG注释鉴定了96个编码参与异黄酮生物合成的七个关键酶的基因。苯丙氨酸氨解酶或查尔酮合酶氨基酸序列的比对揭示了保守的序列，空间结构和活性位点。我们还通过实时定量PCR（qRT-PCR）验证了编码苯丙氨酸氨解酶和查尔酮合酶的关键基因的表达。在异黄酮生物合成途径及其关键酶的这种分析P.玉竹奠定了揭露异黄酮的生物合成的调控机制奠定了基础。