Ovarian cancer is a severe malignant tumour of the female genital organs. Six-transmembrane epithelial antigen of the prostate 1 (STEAP1) expression is correlated with the occurrence and progression of multiple cancers. Here, we assessed STEAP1 expression in ovarian cancer and explored the relationship between STEAP1 and ovarian cancer progression. We used immunohistochemistry and public databases to test STEAP1 expression in normal human ovarian tissues, benign ovarian tumours, and ovarian cancer. The expression of STEAP1 and epithelial-to-mesenchymal transition (EMT)-related genes was analysed using immunocytochemistry, quantitative reverse transcription polymerase chain reaction, and western blotting in ovarian cancer cell lines. Lentivirus was used to knockdown and overexpress STEAP1. Invasion, migration, growth, clonogenicity, and apoptosis were assessed using transwell assay, growth curve, plate clone formation assay, and flow cytometry. We used a tumour xenograft to verify the relationship between STEAP1 and in vivo ovarian cancer cell growth. Matrix metalloproteinase-2 (MMP2) and matrix metalloproteinase-9 (MMP9) activities were examined using Matrix metalloproteinase zymography assay. STEAP1 was highly expressed in the human ovarian cancer tissues and a highly invasive ovarian cancer cell line. Overexpression of STEAP1 was related to poor prognosis in ovarian cancer patients. Down-regulation of STEAP1 suppressed the invasion, migration, proliferation, clonogenicity, EMT progression in human ovarian cancer cells and xenograft tumour growth in vivo, but it enhanced apoptosis. In human ovarian cancer, the STEAP1 gene is highly expressed, and its function is correlated with human ovarian cancer cell metastasis and growth. STEAP1 may be a possible target for suppressing ovarian cancer metastasis.

中文翻译：

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前列腺素1表达的六跨膜上皮抗原通过辅助上皮向间充质的转变而促进卵巢癌转移。

卵巢癌是女性生殖器官的严重恶性肿瘤。前列腺1的六跨膜上皮抗原（STEAP1）的表达与多种癌症的发生和发展相关。在这里，我们评估了STEAP1在卵巢癌中的表达，并探讨了STEAP1与卵巢癌进展之间的关系。我们使用免疫组化和公共数据库来测试STEAP1在正常人卵巢组织，良性卵巢肿瘤和卵巢癌中的表达。使用免疫细胞化学，定量逆转录聚合酶链反应和免疫印迹在卵巢癌细胞系中分析了STEAP1和上皮-间充质转化（EMT）相关基因的表达。慢病毒被用于敲低和过度表达STEAP1。入侵，迁移，生长，克隆性，使用transwell测定，生长曲线，平板克隆形成测定和流式细胞术评估细胞凋亡和凋亡。我们使用肿瘤异种移植物来验证STEAP1与体内卵巢癌细胞生长之间的关系。基质金属蛋白酶2（MMP2）和基质金属蛋白酶9（MMP9）的活性用基质金属蛋白酶酶谱法检测。STEAP1在人卵巢癌组织和高侵袭性卵巢癌细胞系中高表达。在卵巢癌患者中，STEAP1的过表达与预后不良有关。下调STEAP1抑制人卵巢癌细胞的侵袭，迁移，增殖，克隆形成，EMT进展和体内异种移植肿瘤的生长，但它增强了细胞凋亡。在人类卵巢癌中，STEAP1基因高度表达，其功能与人卵巢癌细胞的转移和生长有关。STEAP1可能是抑制卵巢癌转移的可能靶标。