Enzymatic hydrolysis and self-assembly are considered promising methods for preparation of starch nanoparticles (SNPs) because they are environmentally friendly, and time- and cost-effective. These methods are based on the self-assembly of short-chain glucans released from the α-1,6 bonds in amylopectin. Since their discovery, many studies have described the structural and physicochemical properties of self-assembled SNPs. Self-assembled SNPs can be prepared by two methods: using only the soluble portion containing the short-chain glucans, or using the whole hydrolyzate including both insoluble and soluble fractions. Although the structural and physical properties of self-assembled SNPs can be attributed to the composition of the hydrolyzates that participate in self-assembly, this aspect has not yet been discussed. This review focuses on SNPs self-assembled with only soluble short-chain glucans and addresses their characteristics, including formation mechanisms as well as structural and physicochemical properties, compared with SNPs prepared with total hydrolyzates.

中文翻译：

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通过短链葡聚糖酶水解和自组装制备的淀粉纳米颗粒。

酶促水解和自组装被认为是制备淀粉纳米颗粒（SNP）的有前途的方法，因为它们环保且具有时间和成本效益。这些方法基于支链淀粉中α-1,6键释放的短链葡聚糖的自组装。自发现以来，许多研究已经描述了自组装SNP的结构和物理化学性质。自组装SNP可以通过两种方法制备：仅使用含有短链葡聚糖的可溶部分，或使用包括不溶部分和可溶部分的全部水解产物。尽管自组装SNP的结构和物理性质可以归因于参与自组装的水解产物的组成，但尚未讨论此方面。