Application of sodium hydrosulfite along with NaOH successfully reduces and solubilizes all vat dyes at specific temperature. However, being a highly toxic chemical, use of hydrosulfite has raised tremendous ecological concern. In this study, attempts were made to dye cotton with alkaline lipase along with iron (II) salt to study if unstable and highly toxic sodium hydrosulfite could be replaced with it. A 43 Box-Behnken design was run for statistical analysis to obtain optimum parameters for dyeing of cotton using alkaline lipase. The results showed that alkaline lipase along with iron (II) salt was Quite effective and capable of developing comparable dyebath potential, dye receptivity on cotton with comparable dye strength and color fastness as obtained in sodium hydrosulfite based reducing system.

将亚硫酸氢钠与NaOH一起使用可在特定温度下成功还原并溶解所有还原染料。但是，作为一种剧毒化学品，连二亚硫酸盐的使用引起了极大的生态关注。在这项研究中，尝试用碱性脂肪酶和铁（II）盐对棉进行染色，以研究是否可以用它代替不稳定和剧毒的亚硫酸氢钠。运行43 Box-Behnken设计进行统计分析，以获得使用碱性脂肪酶染色棉的最佳参数。结果表明，碱性脂肪酶与铁（II）盐一起使用非常有效，能够在亚硫酸氢钠基还原体系中获得相当的染浴潜力，对棉的染料接受性，相当的染料强度和色牢度。