Pennisetum sinese Roxb (P. sinese) is an efficient and economic energy crop for its high productivity, and has been well studied in its application in phytoremediation and fodder production. However, little is known about how P. sinese plantation and fermented manures of P. sinese-feed livestock affect the composition of soil bacterial and fungal communities. In this study, 16S rRNA/ITS1 gene-based Illumina Miseq sequencing was employed to compare the bacterial and fungal community structure among soils that had been subjected to uncultivated control (CK), 2-year P. sinese plantation (P), and P. sinese plantation combined with the use of organic manures (P-OM) in a "P. sinese-breeding industry" ecological agriculture farm. The results found microbial communities were altered by P. sinese plantation and fertilization. The P. sinese plantation resulted in increased Actinobacteria and Planctomycetes abundance. Comparatively, significant increased abundance of Chloroflexi, Firmicutes, Nitrospirae, and Euryarchaeota, and genes related with nitrogen and carbon metabolic pathways based on PICRUSt prediction was observed in P-OM soil. Fungal compositions suggested a markedly increased abundance of Ascomycota in P soil. Potential organic matter decomposers Candida, Thermoascus, and Aspergillus were enriched in P soil, indicating the enhanced role of fungi in litter decomposition. Redundancy analysis suggested that soil properties (NH4+-N, total nitrogen, organic matter content, and soil water content) significantly correlated with the changes of microbial compositions (P < 0.05). These results highlight the divergence of microbial communities occurs during P. sinese-based plantation, implying functional diversification of soil ecosystem in P. sinese fields.

中文翻译：

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高产草狼尾草Roxb人工林和有机肥改变了生态农业农场中细菌和真菌的群落结构。

狼尾草Roxb（P. sinese）具有高生产力，是一种高效，经济的能源作物，在植物修复和饲料生产中的应用已受到了广泛研究。然而，关于中华假单胞菌人工林和饲喂中华假单胞菌的牲畜的发酵肥料如何影响土壤细菌和真菌群落组成的了解甚少。在这项研究中，基于16S rRNA / ITS1基因的Illumina Miseq测序被用于比较未经耕种的对照（CK），两年的中华假单胞菌（P）和P的土壤中细菌和真菌的群落结构。 。在“中华假种养殖业”的生态农业农场中，使用人工造林结合有机肥（P-OM）。结果发现，中华绒螯蟹的种植和施肥改变了微生物群落。P. 人工林的种植导致放线菌和浮游菌的丰度增加。相比之下，在P-OM土壤中观察到了绿叶弯曲菌，沙门氏菌，硝化螺旋藻和Euryarchaeota的丰度显着增加，并且基于PICRUSt预测发现了与氮和碳代谢途径相关的基因。真菌成分表明，P土中子囊菌的丰度显着增加。潜在的有机物分解物假丝酵母，热曲霉和曲霉富含磷土壤，表明真菌在凋落物分解中的作用增强。冗余分析表明，土壤性质（NH4 + -N，总氮，有机质含量和土壤含水量）与微生物组成的变化显着相关（P <0.05）。这些结果凸显了P期间发生的微生物群落的差异。