The Arabidopsis malonyl-CoA synthetase gene AAE13.1 (AtAAE13.1) plays important roles in cell metabolism, plant growth and development, and environmental stress responses. However, function of AtAAE13.1 in NaCl stress tolerance is not determined yet. Cell suspension cultures of different plant species including rice (Oryza sativa L.), cotton (Gossypium hirsutum L.), and white pine (Pinus strobus L.) were transformed using Agrobacterium tumefaciens strain GV3101 harboring pBI-AtAAE13.1. After confirmation of integration of the AtAAE13.1 gene into the genome by polymerase chain reaction (PCR), Southern and northern blot analyses, NaCl stress tolerance was examined in AtAAE13.1 transgenic cells of O. sativa, G. hirsutum, and P. strobus. AtAAE13.1 expression enhanced NaCl stress tolerance by increasing cell viability and growth rate, decreasing lipid peroxidation, increasing the oxidation rate, and elevating the content of amino acids, glycolate, and phosphoglycolate, as well as elevating the amount of sucrose, glucose, and fructose. In rice cells, AtAAE13.1 elevated expression of the Ca²⁺-dependent protein kinase (CPK) genes under NaCl stress. Overexpression of the AtAAE13.1 gene may be useful for engineering NaCl stress tolerance in different plant species.

在拟南芥丙二酰辅酶A合成酶基因AAE13。1（AtAAE13。1）在细胞代谢，植物生长和发育，以及环境应激反应的重要作用。但是，AtAAE13的功能。NaCl的耐应力性尚未确定为1。使用带有pBI-AtAAE13的根癌农杆菌菌株GV3101转化包括稻（Oryza sativa L.），棉花（Gossypium hirsutum L.）和白松（Pinus strobus L.）在内的不同植物物种的细胞悬浮培养物。1个。确认整合后的AtAAE13。通过聚合酶链反应（PCR），Southern和Northern印迹分析将1个基因导入基因组，在AtAAE13中检测NaCl胁迫耐受性。O的1个转基因细胞。苜蓿，ģ。陆地棉和P。爆炸 原子能机构13。1个通过增加细胞活力和生长速率，减少脂质过氧化作用，增加氧化速率并增加氨基酸，乙醇酸和磷酸乙醇酸的含量以及增加蔗糖，葡萄糖和果糖的量，这种表达增强了NaCl胁迫耐受性。在水稻细胞中，AtAAE13。在NaCl胁迫下，Ca ²⁺依赖性蛋白激酶（CPK）基因的1表达升高。AtAAE13的过表达。1个基因可能可用于改造不同植物物种中的NaCl胁迫耐受性。