The antimicrobial activity of fibrous membranes with electrospun polylactide (PLA)/silver nanoparticles (AgNPs) depends on the accessibility of the AgNPs, among other factors. The challenge is therefore to develop a suitable method for loading the AgNPs onto the fibers. In this study, different techniques for determining the efficacy of the membranes in rendering AgNPs available for antimicrobial activity against Gram-negative Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus bacteria are evaluated. Immersion coating, electrospray-coating, and in-situ loading techniques were used to load AgNPs onto hierarchical electrospun PLA fibers, and their antibacterial effect was evaluated based on a qualitative disc diffusion method. The reduction of AgNO₃ (Ag⁺) to AgNPs was carried out through the use of UV irradiation and neem (Azadirachta indica) leaf extracts. The membranes were coated using an electrospray technique resulted in the best antibacterial activity owing to the uniform dispersion of the AgNPs. The AgNPs were also exposed on the surface of the membranes, as confirmed using scanning electron and transmission electron microscopes. An electrospray is, therefore, a promising technique for the preparation of antimicrobial nanofibrous membranes.

带有静电纺丝聚乳酸（PLA）/银纳米颗粒（AgNPs）的纤维膜的抗菌活性取决于AgNPs的可及性，以及其他因素。因此，面临的挑战是开发一种将AgNP负载到纤维上的合适方法。在这项研究中，评估了确定膜的功效的不同技术，这些技术使AgNP可用于针对革兰氏阴性铜绿假单胞菌和革兰氏阳性金黄色葡萄球菌的抗菌活性。使用浸涂，电喷涂和原位加载技术将AgNPs加载到分层电纺PLA纤维上，并基于定性盘扩散法评估其抗菌效果。AgNO的还原₃（AG ⁺）到的AgNPs是通过使用UV照射和印度楝树（的进行印楝）的叶提取物。由于AgNPs的均匀分散，使用电喷雾技术对膜进行涂覆可产生最佳的抗菌活性。如使用扫描电子显微镜和透射电子显微镜所证实的，AgNP也暴露在膜的表面上。因此，电喷雾是制备抗菌纳米纤维膜的有前途的技术。