Endocytosis plays critical roles in cellular processes, including nutrient uptake and signal transduction. Ede1 is an endocytic scaffolding protein that contributes to endocytic site initiation and maturation in yeast. However, the functions of Ede1 in phytopathogenic fungi are not known. Here, we identified functions of FgEde1 (FGSG_05182) in Fusarium graminearum. Deletion of FgEde1 resulted in defects in hyphal growth, conidiation and ascospore development. The FgEde1 deletion mutant showed reduced deoxynivalenol (DON) production and virulence in wheat. Furthermore, the FgEde1 deletion mutant also exhibited increased resistance to osmotic and oxidative stress as well as cell-wall perturbing agents. Importantly, deletion of FgEde1 increased the severity of autophagy in hyphae. Taken together, these results reveal that FgEde1 is involved in hyphal growth, asexual and sexual reproduction, virulence, stress responses, and autophagy in F. graminearum.

中文翻译：

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内吞 FgEde1 调节禾谷镰刀菌的毒力和自噬。

内吞作用在细胞过程中起着关键作用，包括营养吸收和信号转导。Ede1 是一种内吞支架蛋白，有助于酵母中的内吞位点起始和成熟。然而，Ede1 在植物病原真菌中的功能尚不清楚。在这里，我们确定了 FgEde1 (FGSG_05182) 在禾谷镰刀菌中的功能。FgEde1 的缺失导致菌丝生长、分生孢子和子囊孢子发育缺陷。FgEde1 缺失突变体在小麦中表现出降低的脱氧雪腐镰刀菌烯醇 (DON) 的产生和毒力。此外，FgEde1 缺失突变体还表现出对渗透和氧化应激以及细胞壁干扰剂的抵抗力增加。重要的是，FgEde1 的缺失增加了菌丝自噬的严重程度。综合起来，