This study details the molecular identification of the parasite Neoechinorhynchus rutili diagnosed in fish (Capoeta barroisi, Cyprinus carpio, Barbus rajanorum) caught in Menzelet Dam Lake in Kahramanmaras Province, Turkey. Parasite samples were obtained from the intestines of fish caught from January to June of 2013. The collected parasites were stored in sample vials containing 70% alcohol. Using staining methods and based on morphology, 120 N. rutili specimens were identified. DNA isolation of N. rutili was accomplished using special tissue sets for parasites. Specific primers were utilized in the molecular identification of N. rutili using polymerase chain reaction (PCR) and it was possible to verify that all of the parasites contained N. rutili molecules. In conclusion, using multiple methods we successfully identified and confirmed the presence of N. rutili parasites in the fish caught in Kahramanmaras Province. The process of identification of N. rutili using morphology and staining methods is time-consuming; however, PCR was successfully performed in a short time to accomplish the same results. The success of this study may lead to more original and extensive work aimed at the efficient molecular identification of parasitic agents found in fish.

这项研究详细介绍了在土耳其卡赫拉曼马拉斯省Menzelet水坝湖中捕获的鱼类（Capoeta barroisi，Cyprinus carpio，Barbus rajanorum）中诊断出的寄生虫Neoechinorhynchus rut​​ili的分子鉴定。寄生虫样品是从2013年1月至6月捕获的鱼肠中获得的。收集到的寄生虫存储在含70％酒精的样品瓶中。使用染色方法并基于形态学，鉴定出120个罗非鱼标本。使用特殊的寄生生物组织完成了罗勒氏猪笼草的DNA分离。特异的引物被用于罗非鱼的分子鉴定使用聚合酶链反应（PCR），可以验证所有寄生虫均含有罗非鱼分子。总之，使用多种方法，我们成功地识别并确认了在Kahramanmaras省捕捞的鱼类中存在R. rutili寄生虫。使用形态学和染色方法鉴定罗非鱼的过程很费时；但是，PCR在短时间内成功完成了相同的结果。这项研究的成功可能导致旨在有效鉴定鱼类中寄生虫的分子的更多新颖而广泛的工作。