The determination of benzodiazepines (carbamazepine and clobazam) in rabbit carcass tissues and larvae of three Calliphorid flies is described. After feeding the rabbits with lethal and toxic doses, samples of larvae and carcass tissues were studied. Residual drugs were determined using Ultra-high-performance liquid chromatography – quadrupole time-of-flight mass spectrometry (UHPLC/QTOF-MS). Benzodiazepines and its main active metabolites have been detected in the rabbit tissues at different retention times depending on the dosage used (lethal or toxic). A total of 1150 insects were collected and 800 larvae of the flies Chrysomya albiceps, Lucilia sericata and L. silvarum were used in the analysis. The presence of benzodiazepines in the rabbit tissues has been shown to typically affect the larval development cycle of the three necrophagous flies. Chrysomya albiceps larvae feed on drugs developed faster, while the development of L. sericata and L. silvarum larvae slowed. These results indicate that drugs have an impact on the life cycles of insects, which suggests that the presence of these substances is a factor that needs to be taken into account when estimating the post-mortem interval (PMI).

描述了三种Call体蝇的家畜尸体组织和幼虫中苯二氮卓类药物（卡马西平和氯巴嗪）的测定。在给兔子喂食致死剂量和中毒剂量后，对幼虫和car体组织样本进行了研究。使用超高效液相色谱-四极杆飞行时间质谱（UHPLC / QTOF-MS）测定残留药物。根据所用剂量（致死或有毒），已在兔子组织中以不同的保留时间检测到了苯二氮卓类及其主要活性代谢物。总共收集了1150种昆虫和800只苍蝇蝇幼虫（Chrysomya albiceps），Luc蝇（Lucilia sericata）和乳杆菌L. silvarum被用于分析。兔子组织中苯二氮卓类药物的存在已显示出通常会影响三种食尸蝇的幼虫发育周期。以白屈菜为食的幼虫以药物为食的发育较快，而丝角线虫和silvarum幼虫的发育速度减慢。这些结果表明药物对昆虫的生命周期有影响，这表明在估计验尸间隔（PMI）时需要考虑这些物质的存在。