Abstract We have previously developed a poly(L-lactic) acid (PLLA) bioengineered anterior cruciate ligament (ACL) matrix that has demonstrated enhanced healing when seeded with primary ACL cells prior to implantation in a rabbit model, as compared with the matrix alone. This suggests that improving cell adhesion on the matrix may beneficially affect the healing response and long-term performance of the bioengineered ACL matrix. One regenerative engineering approach involves enhancing the surface properties of the matrix to support cell adhesion and growth in combination with point-of-care stem cell therapy. Herein, we studied the cell adhesion properties of PLLA braided microfiber matrices enhanced through the physical adsorption of fibronectin and air plasma treatment. We evaluated the kinetics and binding efficiency of fibronectin onto matrices at three time points and three fibronectin concentrations. Incubating the matrix for 120 min in a solution of 25 µg·mL−1 fibronectin achieved the greatest binding efficiency to the matrix and cellular adhesion. Exposing the matrices to air plasma treatment for 5 min before fibronectin adsorption significantly enhanced the cell adhesion of rabbit bone marrow-derived mesenchymal stem cells (R-BMMSCs) 24 h post cell seeding. Finally, cellular proliferation was monitored for up to 21 d, the matrices were exposed to air plasma treatment, and fibronectin adsorption was found to result in enhanced cell number. These findings suggest that exposure to air plasma treatment and fibronectin adsorption enhances the cellular adhesion of PLLA braided microfiber matrices and may improve the clinical efficacy of the matrix in combination with point-of-care stem cell therapies.

中文翻译：

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增强生物工程前交叉韧带基质的表面特性，用于即时干细胞治疗

摘要我们以前开发了一种聚（L-乳酸）酸（PLLA）生物工程前交叉韧带（ACL）基质，与单独的基质相比，在植入兔模型之前用原代 ACL 细胞接种时，该基质已证明可以增强愈合。这表明，改善基质上的细胞粘附可能有益地影响生物工程 ACL 基质的愈合反应和长期性能。一种再生工程方法涉及增强基质的表面特性，以支持细胞粘附和生长，并结合即时干细胞疗法。在此，我们研究了通过纤连蛋白的物理吸附和空气等离子体处理增强的 PLLA 编织超细纤维基质的细胞粘附特性。我们在三个时间点和三个纤连蛋白浓度评估了纤连蛋白在基质上的动力学和结合效率。将基质在 25 µg·mL-1 纤连蛋白溶液中孵育 120 分钟可实现与基质和细胞粘附的最大结合效率。在细胞接种后 24 小时，在纤连蛋白吸附前将基质暴露于空气等离子体处理 5 分钟，显着增强了兔骨髓间充质干细胞 (R-BMMSCs) 的细胞粘附。最后，监测细胞增殖长达 21 天，将基质暴露于空气等离子体处理，发现纤连蛋白吸附导致细胞数量增加。