In the present study, light was found to significantly increase the total fatty acid and carotenoid content in Crypthecodinium sp. SUN, and the levels of increase were significantly higher than those in Crypthecodinium cohnii. To elucidate the underlying mechanism, comparative transcriptome analysis was firstly conducted in C. sp. SUN, a total of 62,393 genes were obtained, and three functional gene classifications, chemotactic activity, rhythm process, and cell killing, were present in C. sp. SUN but not C. cohnii. Further, 663 transcription factors, distributed across 26 transcription factors families, were predicted in C. sp. SUN, accounting for 1.09% of the total number of genes. In addition, the non-mevalonate, mevalonate, and carotenoid biosynthesis pathways were all up-regulated under light conditions, and light may promote lipid accumulation by elevating diacylglycerol and triacylglycerol synthesis and inhibiting their degradation. Finally, co-expression network analysis revealed that cytochrome c oxidase, cryptochrome, and a PhD zinc finger transcription factor may play key regulatory roles in light induced behaviors of C. sp. SUN. In conclusion, this study explored the mechanisms underlying light induction in the strict heterotrophic microalga, C. sp. SUN, identifying potential regulatory pathways and factors involved in this process.

在本研究中，发现光显着增加了Crypthecodinium sp。中的总脂肪酸和类胡萝卜素含量。SUN，并且升高的水平显着高于Crypthecodinium cohnii。为了阐明其潜在机制，首先在C. sp。中进行了比较转录组分析。SUN，共获得的62393个基因，和三个功能基因分类，趋化活性，节奏过程，和细胞杀伤，存在于C.藻。SUN但不是C. cohnii。此外，在C中预测了分布在26个转录因子家族中的663个转录因子。sp。SUN，占基因总数的1.09％。此外，非甲羟戊酸，甲羟戊酸和类胡萝卜素的生物合成途径均在光照条件下上调，并且光照可能通过提高二酰基甘油和三酰基甘油的合成并抑制其降解来促进脂质蓄积。最后，共表达网络分析表明，细胞色素c氧化酶，隐色染料和PhD锌指转录因子可能在C. sp。的光诱导行为中起关键调节作用。太阳。总之，本研究探讨了严格异养微藻C. sp。中光诱导的潜在机制。SUN，确定此过程涉及的潜在监管途径和因素。