Membrane curvature plays a pivotal role in cellular life, including cellular uptake and membrane trafficking. The modulation of membrane curvature provides a novel means of manipulating cellular events. In this report, we show that a nine-residue amphiphilic peptide (R6W3) stimulates endocytic uptake by inducing membrane curvature. Curvature formation on cell membranes was confirmed by observing the cellular distribution of the curvature-sensing protein amphiphysin fused with a yellow fluorescent protein (Amp-YFP). Dot-like signals of Amp-YFP were visible following the addition of R6W3, suggesting curvature formation in cell membranes, leading to endocytic cup and vesicle formation. The promotion of endocytic uptake was confirmed using the endocytosis marker polydextran. Treatment of cells with R6W3 yielded a 4-fold dextran uptake compared with untreated cells. The amphiphilic helical structure of R6W3 was also crucial for R6W3-stimulated endocytic uptake.

中文翻译：

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人工两亲性肽通过诱导膜曲率促进内吞摄取。

膜曲率在细胞生命中起着关键作用，包括细胞摄取和膜运输。膜曲率的调节提供了一种操纵细胞事件的新颖手段。在此报告中，我们显示了九个残基的两亲肽（R6W3）通过诱导膜曲率刺激内吞摄取。通过观察与黄色荧光蛋白（Amp-YFP）融合的曲率敏感蛋白两性蛋白的细胞分布，可以确认细胞膜上的曲率形成。加入R6W3后，可以看到Amp-YFP的点状信号，表明细胞膜中形成了曲率，从而导致了内吞杯和囊泡的形成。使用内吞标志物聚葡聚糖证实了内吞摄取的促进。与未处理的细胞相比，用R6W3处理的细胞吸收了4倍的葡聚糖。R6W3的两亲性螺旋结构对于R6W3刺激的内吞摄取也至关重要。