The chemistry of diazo compounds has generated a huge breadth of applications in the field of organic synthesis. Their versatility combined with their tunable reactivity, stability, and chemoselectivity makes diazo compounds desirable reagents for chemical biologists. Here, we describe a method for the precise installation of diazo handles on proteins and antibodies in a mild and specific approach. Subsequent 1,3-cycloaddition reactions with strained alkynes enable both bioimaging through an in-cell “click” reaction and probing of the cysteine proteome in cell lysates. The selectivity and efficiency of these processes makes these suitable reagents for chemical biology studies.

中文翻译：

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在蛋白质上精确安装重氮标记的侧链，以实现体外和细胞内特定部位标记。

重氮化合物的化学作用已在有机合成领域产生了广泛的应用。它们的多功能性以及可调的反应性，稳定性和化学选择性使重氮化合物成为化学生物学家的理想试剂。在这里，我们描述了一种以温和而特定的方法在蛋白质和抗体上精确安装重氮手柄的方法。随后的带有应变炔烃的1,3-环加成反应既可以通过细胞内“点击”反应进行生物成像，也可以探测细胞裂解物中的半胱氨酸蛋白质组。这些过程的选择性和效率使这些试剂适合用于化学生物学研究。