In flowering plants, pollen development is under a dynamic and well‐orchestrated transcriptional control, characterized by an early phase with high transcript diversity and a late post‐mitotic phase skewed to a cell‐type‐specific transcriptome. Such transcriptional changes require a balance between synthesis and degradation of mRNA transcripts, the latter being initiated by deadenylation. The CCR4‐NOT complex is the main evolutionary conserved deadenylase complex in eukaryotes, and its function is essential during germline specification in animals. We hypothesized that the CCR4‐NOT complex might play a central role in mRNA turnover during microgametogenesis in Arabidopsis. Disruption of NOT1 gene, which encodes the scaffold protein of the CCR4‐NOT complex, showed abnormal seed set. Genetic analysis failed to recover homozygous progeny, and reciprocal crosses confirmed reduced transmission through the male and female gametophytes. Concordantly, not1 embryo sacs showed delayed development and defects in embryogenesis. not1 pollen grains exhibited abnormal male germ unit configurations and failed to germinate. Transcriptome analysis of pollen from not1/+ mutants revealed that lack of NOT1 leads to an extensive transcriptional deregulation during microgametogenesis. Therefore, our work establishes NOT1 as an important player during gametophyte development in Arabidopsis.

中文翻译：

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拟南芥中配子体发育需要AtNOT1。

在开花植物中，花粉发育处于动态和精心安排的转录控制之下，其特征是早期具有较高的转录本多样性，而有丝分裂后后期则偏向特定于细胞类型的转录组。此类转录变化需要在mRNA转录物的合成与降解之间取得平衡，后者是由去腺苷酸化作用引发的。CCR4-NOT复合物是真核生物中主要的进化保守的腺苷酸酶复合物，其功能在动物生殖系鉴定过程中至关重要。我们假设CCR4-NOT复合物可能在拟南芥微配子发生过程中的mRNA转换中发挥重要作用。NOT1中断编码CCR4-NOT复合物支架蛋白的基因显示出异常的结实。遗传分析未能恢复纯合子代，并且相互杂交证实了通过雌雄配子体的传播减少。一致地，not1胚囊显示出延迟的发育和胚胎发生中的缺陷。not1花粉粒表现出异常的雄性生殖单位构型，并且无法发芽。来自not1 / +突变体的花粉的转录组分析显示，NOT1的缺乏导致微配子发生过程中广泛的转录失调。因此，我们的工作确立了NOT1在拟南芥配子体发育过程中的重要作用。