ABSTRACT

The objective of this study was to establish a real-time LAMP assay for authentication of rape (Brassica napus) honey to protect consumers from commercial honey adulteration. The LAMP primers targeting the internal transcribed spacer (ITS) of Brassica napus were designed, and its specificity was tested. The LAMP reaction temperature was also optimized, and the detection limit of the LAMP assay was determined with a serial dilution of genomic DNA from the seeds of Brassica napus. The results showed that the real-time LAMP assay can accurately and specifically detect the rape component in honey, and the detection limit was 10 pg genomic DNA of Brassica napus. Data on monofloral honey samples indicate that the real-time LAMP assay was 100% in concordance with the reported TaqMan^TM PCR assay. This study provides a promising solution for facilitating the authentication of rape honey in food retail market.

摘要

这项研究的目的是建立一个实时的LAMP测定法，以鉴定强奸（Brassica napus）蜂蜜，以保护消费者免受商业蜂蜜掺假的侵害。设计了针对甘蓝型油菜内部转录间隔区（ITS）的LAMP引物，并测试了其特异性。还优化了LAMP反应温度，并用来自甘蓝型油菜种子的基因组DNA的系列稀释液确定了LAMP测定的检测限。结果表明，实时LAMP法可以准确，特异性地检测出蜂蜜中的油菜成分，其检测限为甘蓝型油菜的10 pg基因组DNA。。单花蜂蜜样品的数据表明，实时的LAMP测定与报道的TaqMan ^TM PCR测定一致，为100％。该研究为促进食品零售市场中强奸蜂蜜的认证提供了有希望的解决方案。