当前位置: X-MOL 学术3 Biotech › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Role of hydrogen cyanamide (HC) in grape bud dormancy release: proteomic approach.
3 Biotech ( IF 2.8 ) Pub Date : 2020-05-05 , DOI: 10.1007/s13205-020-02194-5
Muhammad Khalil-Ur-Rehman 1, 2 , Wu Wang 1 , Huan Zheng 1 , Muhammad Faheem 3 , Shahid Iqbal 1 , Zhen Guo Shen 2 , Jianmin Tao 1
Affiliation  

In the present study, we identified changes in protein expression patterns of grapevine buds when treated with hydrogen cyanamide (HC). HC induced a shift of more than 2-folds in the expression of 1250 proteins out of approximately 7000 detected proteins. The majority of the differentially expressed proteins (DEPs) were localized in the chloroplast (419) and cytoplasm (347). Most of the detected DEPs were linked with energy metabolism, redox activity, hormone, and stress signaling. Particularly, the DEPs associated with defense and sugar metabolism showed significantly higher expression in HC-treated buds. Kyoto encyclopedia of genes and genomes (KEGG) analysis revealed significant enrichment for circadian rhythm, ribosome, and metabolic pathways. Moreover, the antioxidant activity of peroxidase (POD) increased at initial stages but declined at later stages (18 days post-treatment). This study identified several dormancy-related proteins that regulated signaling, as well as metabolic pathways upon HC application. The outcome of this study provides insights into the role of HC in dormancy release for grapevine production, hence useful to alleviate yield losses in mild winter regions.

中文翻译:

氰氨化氢(HC)在葡萄芽休眠释放中的作用:蛋白质组学方法。

在本研究中,我们确定了用氰氨氢(HC)处理后葡萄芽中蛋白质表达模式的变化。HC诱导了大约7000种检测到的蛋白质中1250种蛋白质表达的超过2倍的偏移。大多数差异表达蛋白(DEP)位于叶绿体(419)和细胞质(347)中。大多数检测到的DEP与能量代谢,氧化还原活性,激素和应激信号有关。特别是,与防御和糖代谢相关的DEP在HC处理的芽中显示出明显更高的表达。《京都议定书》的基因和基因组百科全书(KEGG)分析显示,昼夜节律,核糖体和代谢途径显着丰富。此外,过氧化物酶(POD)的抗氧化活性在初期增加,但在后期(治疗后18天)下降。这项研究确定了几种休眠相关蛋白,这些蛋白可调节信号传导以及施用HC后的代谢途径。这项研究的结果提供了关于HC在休眠释放中对葡萄生产的作用的见解,因此有助于减轻冬季温和地区的单产损失。
更新日期:2020-05-05
down
wechat
bug