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Biomarkers of Planarian Dugesia japonica in Response to Herbicide Glyphosate Exposure.
Bulletin of Environmental Contamination and Toxicology ( IF 2.7 ) Pub Date : 2020-05-05 , DOI: 10.1007/s00128-020-02865-2
He-Cai Zhang 1 , Chang-Ying Shi 1 , Yu-Juan Yang 1 , Guang-Wen Chen 1 , De-Zeng Liu 1
Affiliation  

As the worldwide top-selling herbicide, glyphosate is ubiquitously distributed in the natural environment, and its influence on the ecological safety and human health has being increasingly concerned. In this study, mRNA expressions of GPX and three heat shock protein genes in freshwater planarian Dugesia japonica in response to glyphosate were determined, and two oxidative stress parameters were measured. The results suggested that GPX activity can be used as a more sensitive biomarker in contrast with GPX gene expression, and mRNA expressions of Hsp70, Hsp90 genes are more sensitive than Hsp40 for planarians in response to glyphosate stress. Besides, the deduced T-AOC as well as varied GPX activity and mRNA expression levels of Hsps also indicated that glyphosate exposure would inhibit antioxidation and induce oxidative stress in D. japonica, while specific antioxidant systems and stress proteins tried to protect cells by their own regulation. The results of this study will be helpful to elucidate the stress response mechanisms of freshwater planarians to herbicide glyphosate.

中文翻译:

响应于除草剂草甘膦暴露的Planarian Dugesia japonica的生物标记。

草甘膦作为全球最畅销的除草剂,广泛分布于自然环境中,其对生态安全和人类健康的影响日益受到关注。本研究测定了草甘膦对淡水planar日本DGPX和三个热休克蛋白基因的mRNA表达,并测定了两个氧化应激参数。结果表明,与GPX基因表达相比,GPX活性可以作为更敏感的生物标志物,Hsp70Hsp90基因的mRNA表达比Hsp40更敏感。应对草甘膦胁迫的平面动物。另外,推导的T-AOC以及多种多样GPX活性和mRNA的表达水平的Hsp还表明,草甘膦曝光将抑制抗氧化和诱导的氧化应激中d粳稻,而特定的抗氧化剂系统和应激蛋白则试图通过自身调节来保护细胞。这项研究的结果将有助于阐明淡水刨刀对除草剂草甘膦的胁迫响应机制。
更新日期:2020-05-05
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