Lacaziosis is a cutaneous chronic mycosis caused by Lacazia loboi. Macrophages are important cells in the host immune response in fungal infections. The macrophage population exhibits strong plasticity that varies according to the stimuli in the microenvironment of lesions M1 profile promotes a Th1 pattern of cytokines and a microbicidal function and M2 is related to Th2 cytokines and immunomodulatory response. We investigated the population of M1 and M2 polarized macrophages in human cutaneous lesions. A total of 27 biopsies from human lesions were submitted to an immunohistochemistry protocol using antibodies to detect M1 and M2 macrophages (Arginase-1, CD163, iNOS, RBP-J and cMAF). We could observe high number of cells expressing Arginase1, CD163 and c-MAF that correspond to elements of the M2 profile of macrophage, over iNOS and RBP-J (elements of the M1 profile). The results suggest a predominant phenotype of M2 macrophages, which have an immunomodulatory role and probably contributing to chronicity of Lacaziosis.

中文翻译：

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M2极化的巨噬细胞可确定人在唇裂中的皮肤病变。

Lacaziosis是由Lacazia loboi引起的皮肤慢性真菌病。巨噬细胞是真菌感染宿主免疫反应中的重要细胞。巨噬细胞群体表现出强大的可塑性，其根据病变的微环境中的刺激而变化。M1谱促进Th1型细胞因子和杀菌功能，M2与Th2型细胞因子和免疫调节反应有关。我们调查了人类皮肤病变中M1和M2极化巨噬细胞的种群。使用抗体检测M1和M2巨噬细胞（Arginase-1，CD163，iNOS，RBP-J和cMAF）的抗体，对来自人类病变的总共27份活检进行了免疫组织化学实验。我们可以观察到大量表达Arginase1，CD163和c-MAF的细胞，它们对应于巨噬细胞M2谱的元素，通过iNOS和RBP-J（M1配置文件的元素）。结果表明M2巨噬细胞的主要表型，具有免疫调节作用，并可能有助于Lacaziosis的慢性。