This study aimed to describe an efficient method for optimized synthesis of gold nanoparticles (Au NPs) by using Eryngium bungei (EB) hydro-alcoholic extract and also to demonstrate its hepatoprotective activity in comparison with a chemically synthesized counterpart in a rat model of acetaminophen-induced acute liver injury. To this end, various parameters were optimized in 12 trials to enhance the yield of gold nanoparticles (Au NPs) synthesized at room temperature. The synthesized NPs were characterized by different methods. In hepatoprotective study, effect of pretreatment with these Au NPs at 100, 200 and 400 mg/kg in comparison with chemically synthesized Au NPs was evaluated in rats with acetaminophen-induced liver injury. The optimal conditions were HAuCl₄ at 1 mM, a reaction time of 4 h with 1:9 ratio of plant extract to HAuCl₄ which yielded spherical NPs in transmission electron microscopy (TEM) with a size of 53.8 nm in dynamic light scattering (DLS) analysis. The Fourier-transform infrared spectroscopy (FTIR) spectrum of the Au NPs was relatively similar to the extract that indicated various types of functional groups. Pretreatment with Au NPs ameliorated acetaminophen-induced liver injury based on liver function tests. For oxidative stress parameters and pro inflammatory cytokines, the effect of green synthesized Au NPs was more profound than chemically synthesized NPs. In conclusion, the hydro-alcoholic extract from aerial parts of EB can be successfully used for synthesizing Au NPs. Green synthesized Au NPs show more profound anti-oxidant and anti-inflammatory effects than their chemically synthesized counterpart in a rat model of acute liver injury.

这项研究旨在描述一种有效的方法，该方法通过使用布氏刺槐（EB）水醇提取物优化金纳米颗粒（Au NPs）的最佳合成，并与对乙酰氨基酚-大鼠模型中化学合成的对等物相比，证明其对肝脏具有保护作用。诱发急性肝损伤。为此，在12个试验中优化了各种参数，以提高室温下合成的金纳米颗粒（Au NPs）的产率。合成的NPs用不同的方法表征。在肝保护研究中，在对乙酰氨基酚诱发的肝损伤大鼠中，与化学合成的金纳米颗粒相比，以100、200和400 mg / kg的金纳米颗粒进行预处理的效果进行了评估。最佳条件为HAuCl ₄在1 mM下，反应时间为4小时，植物提取物与HAuCl _4的比例为1：9在透射电子显微镜（TEM）中通过动态光散射（DLS）分析得到了球形的NP，尺寸为53.8 nm。Au NPs的傅立叶变换红外光谱（FTIR）光谱与提取物相对相似，后者表明各种类型的官能团。基于肝功能测试，用金纳米颗粒进行的预处理可减轻对乙酰氨基酚引起的肝损伤。对于氧化应激参数和促炎性细胞因子，绿色合成的金纳米颗粒的作用比化学合成的纳米颗粒更深远。总之，从EB地上部分提取的水-醇提取物可以成功地用于合成金纳米颗粒。在大鼠急性肝损伤模型中，绿色合成的金纳米颗粒比其化学合成的对应物显示出更深刻的抗氧化和抗炎作用。