Past research has sought to improve the production of cyclopropane fatty acids by the oleaginous yeast Yarrowia lipolytica by heterologously expressing the E. coli fatty acid synthase gene and improving cultivation processes. Cyclopropane fatty acids display properties that hold promise for biofuel applications. The E. coli fatty acid synthase gene was introduced into several genetic backgrounds of the yeast Y. lipolytica to optimize lipid synthesis; the mean cyclopropane fatty acid productivity was 43 mg L-1 h-1 on glucose, and the production rate reached its maximum (3.06 g L-1) after 72 h of cultivation in a bioreactor. The best strain (JMY6851) overexpressed simultaneously the E. coli cyclopropane fatty acid synthase gene under a hybrid promoter (hp8d) and Y. lipolytica LRO1 gene. In fed-batch process using crude glycerol as carbon source, JMY6851 strain displayed high lipid accumulation (78% of dry cell weight) and high biomass production (56 g L-1). After 165 h of cultivation, cyclopropane fatty acids represented 22% of the lipids produced; cyclopropane fatty acid productivity (103.3 mg L-1 h-1) was maximal at 72.5 h of cultivation.

中文翻译：

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用解脂耶氏酵母生产CFA的发酵过程。

过去的研究试图通过异源表达大肠杆菌脂肪酸合酶基因并改善培养过程来改善油性酵母解脂耶氏酵母（Yarrowia lipolytica）产生的环丙烷脂肪酸。环丙烷脂肪酸具有可用于生物燃料的特性。将大肠杆菌脂肪酸合酶基因引入酵母解脂耶氏酵母的几种遗传背景，以优化脂质合成。在葡萄糖上的平均环丙烷脂肪酸生产率为43 mg L-1 h-1，在生物反应器中培养72 h后，生产率达到最高（3.06 g L-1）。最佳菌株（JMY6851）在杂合启动子（hp8d）和解脂耶氏酵母LRO1基因的同时同时过表达大肠杆菌环丙烷脂肪酸合酶基因。在以粗甘油为碳源的分批补料工艺中，JMY6851菌株显示出高脂质积累（占干细胞重量的78％）和高生物量产生（56 g L-1）。培养165小时后，环丙烷脂肪酸占所产生脂质的22％。培养72.5 h时，环丙烷脂肪酸生产率（103.3 mg L-1 h-1）最高。