In this study, the differential regeneration process in sponge Cinachyrella cf cavernosa (Lamarck, 1815) was examined by creating wounds at basal and apical tissues. The differences in wound healing were investigated based on qualitative and quantitative changes in collagen at both wound locations. The different cell types were also documented. The basal tissue was found to heal faster than apical tissue. Collagen was 21.74% more in regenerating basal tissue than the apical tissue, indicating its involvement in accelerated regeneration. To understand the molecular process of gene regulation during regeneration in basal tissues of C.cf cavernosa, transcriptomic analysis was carried out using Illumina NextSeq 500. From the samples used for RNA-seq, on an average, 35 million reads were obtained. Out of 65587 total Coding DNA Sequences (CDS), 37145 were annotated. The differential gene expression analysis showed upregulation in collagen-related gene Col and actin-related genes during progressive stages of regeneration. Contrary to other metabolism-related genes, lipid metabolism-related genes were expressed in more numbers with consecutive stages of regeneration highlighting their role in stress management of the sponge. This study serves to advance our understanding of the regeneration process, factors affecting the rate of this process and transcriptomic changes in regenerating sponge.

中文翻译：

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基于伤口位置的海绵状Cinachyrella cf海绵体再生进展

在这项研究中，海绵 Cinachyrella cf 海绵体 (Lamarck, 1815) 的差异再生过程通过在基底和顶端组织上创建伤口来检查。基于两个伤口位置胶原蛋白的定性和定量变化研究了伤口愈合的差异。还记录了不同的细胞类型。发现基底组织比顶端组织愈合得更快。再生基底组织中的胶原蛋白比顶端组织多 21.74%，表明其参与了加速再生。为了了解 C.cf 海绵体基底组织再生过程中基因调控的分子过程，使用 Illumina NextSeq 500 进行了转录组学分析。 从用于 RNA-seq 的样本中，平均获得了 3500 万个读数。在 65587 个编码 DNA 序列 (CDS) 中，37145 条注释。差异基因表达分析显示在再生的渐进阶段胶原相关基因 Col 和肌动蛋白相关基因的上调。与其他代谢相关基因相反，脂质代谢相关基因的表达数量更多，连续的再生阶段突出了它们在海绵压力管理中的作用。这项研究有助于促进我们对再生过程、影响再生过程速率的因素和再生海绵转录组变化的理解。脂质代谢相关基因的表达数量更多，连续的再生阶段突出了它们在海绵压力管理中的作用。这项研究有助于促进我们对再生过程、影响再生过程速率的因素和再生海绵转录组变化的理解。脂质代谢相关基因的表达数量更多，连续的再生阶段突出了它们在海绵压力管理中的作用。这项研究有助于促进我们对再生过程、影响再生过程速率的因素和再生海绵转录组变化的理解。