In this study, the controlled synthesis of microcarriers and CpE loading were aimed to achieve by microfluidic system. The effect of temperature on chitosan in the stabilization of silver nanoparticles, microcarrier size and the encapsulation efficiency was measured. Encapsulation was achieved by the controlled rapid mixing in polymethyl-methacrylate (PMMA) microfluidic chip. The CpE was found to contain primary and secondary phenols in the phytochemical screening. The capping property of chitosan was achieved by heating reaction mixture in polytetrafluoroethylene (PTFE) tube to get the stabilized silver nanoparticles which were found to be embedded in chitosan microspheres of size 23.7 µm on average. Fourier-transform infrared (FTIR) and ultraviolet–visible (UV–Vis) spectroscopy results indicated the successful encapsulation of CpE in microcarriers. The average percentage encapsulation efficiency (% EE) of CsAg was found to be 77.125 ± 6.9%. The CpE-loaded microcarriers had shown significant anti-oxidant activity (p < 0.01). CpE-loaded microcarriers were evaluated to have robust cytotoxicity against 4T1 breast cancer cells at very low dose (IC₅₀ = 42.53 µg/mL), which inhibited 95% of cancer cells viability. These results confer that microfluidic system plays an important role in the formation of CpE-loaded CsAg microcarriers that could effectively (p < 0.0001) kill the breast cancer cells.

中文翻译：

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用于将植物提取物封装在壳聚糖微载体中的微流控平台，该载体嵌入银纳米颗粒用于乳腺癌细胞

在这项研究中，微载体系统旨在实现微载体的受控合成和CpE的负载。测量了温度对壳聚糖在银纳米颗粒的稳定化，微载体尺寸和包封效率中的影响。通过在聚甲基丙烯酸甲酯（PMMA）微流控芯片中进行受控快速混合来实现封装。在植物化学筛选中发现CpE包含伯酚和仲酚。壳聚糖的封端性能是通过在聚四氟乙烯（PTFE）管中加热反应混合物以获得稳定的银纳米颗粒来实现的，该纳米颗粒被发现嵌入平均大小为23.7 µm的壳聚糖微球中。傅里叶变换红外（FTIR）和紫外可见（UV-Vis）光谱结果表明CpE成功地封装在微载体中。发现CsAg的平均百分比包封效率（％EE）为77.125±6.9％。载有CpE的微载体显示出显着的抗氧化活性（p  <0.01）。评估了加载CpE的微载体在非常低的剂量（I​​C ₅₀  = 42.53 µg / mL）下对4T1乳腺癌细胞具有强大的细胞毒性，从而抑制了95％的癌细胞活力。这些结果表明，微流体系统在可有效（p  <0.0001）杀死乳腺癌细胞的CpE加载CsAg微载体的形成中起着重要作用。