The lipids produced by oleaginous microbes are considered sustainable resources for biofuels. To facilitate controlled lipid production and lipid analysis, more efficient lipid extraction methods are required. This study describes the automated pressurized liquid extraction (APLE) method for lipid extraction from dried cells of the oleaginous yeast species Rhodosporidium toruloides and Cryptococcus curvatus. Cells were mixed with diatomite in a mortar, added to the sample chamber, and treated with a mixture of chloroform and methanol at 105 °C. More than 95% lipids were extracted. Analysis by using high-performance thin-layer chromatography showed that the neutral lipid contents in the obtained samples by APLE method were similar to those by the ball milling-assisted extraction method. The lipids had an essentially identical fatty acid composition compared with lipids extracted with the acid-heating extraction (AHE) method. This demonstrated that lipids can be efficiently extracted from oleaginous yeasts in less time and without harsh pretreatment procedures.

中文翻译：

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从含油酵母中自动加压液体提取微生物脂质。

油质微生物产生的脂质被认为是生物燃料的可持续资源。为了促进受控的脂质生产和脂质分析，需要更有效的脂质提取方法。这项研究描述了一种自动加压液体提取（APLE）方法，用于从含油酵母物种圆鼻假单胞菌和弯形隐球菌的干细胞中提取脂质。在研钵中将细胞与硅藻土混合，添加到样品室中，并在105°C下用氯仿和甲醇的混合物处理。提取了超过95％的脂质。高效薄层色谱法分析表明，APLE法测定的样品中脂质含量与球磨辅助萃取法相似。与通过酸热提取（AHE）方法提取的脂质相比，这些脂质具有基本相同的脂肪酸组成。这表明脂质可以在更少的时间内从油脂性酵母中高效提取而无需苛刻的预处理程序。