This work intended to prospect new phytase-producing organisms. In silico genomic analyses allowed the selection of twelve potential phytase-producing fungi. Based on gene sequence, it was possible to identify four well-defined groups of phytate-degrading enzymes: esterase-like, β-propeller phytases (βPP), phosphoglycerate mutase-like, and phytases of the histidine acid phosphatases (HAP) family. Analysis of the predicted genes encoding phytases belonging to the HAP family and βPP phytases and in silico characterization of these enzymes indicated divergence among the catalytic activities. Predicted fungal βPP phytases exhibited higher molecular mass (around 77 kDa) probably due to the epidermal growth factor-like domain. Twelve sequences of phytases contained signal peptides, of which seven were classified as HAP and five as βPP phytases, while ten sequences were predicted as phytases secreted by non-classical pathways. These fungi were grown in liquid or semi-solid medium, and the fungal enzymatic extracts were evaluated for their ability to hydrolyze sodium phytate at 50 °C and pH ranging from 2.0 to 9.0. Seven fungi were identified as phytase producers based on phosphate release under enzyme assay conditions. Results obtained from in silico analyses combining experimental enzymatic activities suggest that some selected fungi could secrete βPP phytases and HAP phytases.

中文翻译：

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真菌植酸酶的基因组规模表征以及β-螺旋桨植酸酶和组氨酸酸性磷酸酶之间的比较研究。

这项工作旨在寻找新的植酸酶生产生物。通过计算机基因组分析，可以选择十二种潜在的植酸酶生产真菌。根据基因序列，可以鉴定出四类定义明确的肌醇六磷酸降解酶：酯酶样，β-螺旋桨肌醇六磷酸酶（βPP），磷酸甘油酸突变酶样和组氨酸酸性磷酸酶（HAP）家族的肌醇六磷酸酶。对编码属于HAP家族的植酸酶和βPP植酸酶的预测基因进行分析，并对这些酶进行计算机表征，表明催化活性之间存在差异。预测的真菌βPP植酸酶表现出较高的分子量（约77 kDa），可能是由于表皮生长因子样结构域。十二个肌醇六磷酸酶序列包含信号肽，其中七个被分类为HAP，五个被分类为βPP肌醇六磷酸，而十个序列被预测为非经典途径分泌的植酸酶。这些真菌在液体或半固体培养基中生长，并在50°C和2.0至9.0的pH值下评估了真菌酶提取物水解植酸钠的能力。根据酶测定条件下的磷酸盐释放，确定了七种真菌为植酸酶生产者。计算机分析结合实验酶活性获得的结果表明，某些选定的真菌可以分泌βPP肌醇六磷酸酶和HAP肌醇六磷酸酶。根据酶测定条件下的磷酸盐释放，确定了七种真菌为植酸酶生产者。通过计算机分析结合实验酶活性获得的结果表明，某些选定的真菌可以分泌βPP肌醇六磷酸酶和HAP肌醇六磷酸酶。根据酶测定条件下的磷酸盐释放，确定了七种真菌为植酸酶生产者。计算机分析结合实验酶活性获得的结果表明，某些选定的真菌可以分泌βPP肌醇六磷酸酶和HAP肌醇六磷酸酶。