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Structure of the cytoplasmic ring of the Xenopus laevis nuclear pore complex by cryo-electron microscopy single particle analysis.
Cell Research ( IF 44.1 ) Pub Date : 2020-05-06 , DOI: 10.1038/s41422-020-0319-4
Gaoxingyu Huang 1 , Yanqing Zhang 2, 3 , Xuechen Zhu 2, 3 , Chao Zeng 4 , Qifan Wang 1 , Qiang Zhou 2, 3 , Qinghua Tao 1 , Minhao Liu 1 , Jianlin Lei 1 , Chuangye Yan 1 , Yigong Shi 1, 2, 3, 4
Affiliation  

The nuclear pore complex (NPC) exhibits structural plasticity and has only been characterized at local resolutions of up to 15 Å for the cytoplasmic ring (CR). Here we present a single-particle cryo-electron microscopy (cryo-EM) structure of the CR from Xenopus laevis NPC at average resolutions of 5.5-7.9 Å, with local resolutions reaching 4.5 Å. Improved resolutions allow identification and placement of secondary structural elements in the majority of the CR components. The two Y complexes in each CR subunit interact with each other and associate with those from flanking subunits, forming a circular scaffold. Within each CR subunit, the Nup358-containing region wraps around the stems of both Y complexes, likely stabilizing the scaffold. Nup205 connects the short arms of the two Y complexes and associates with the stem of a neighboring Y complex. The Nup214-containing region uses an extended coiled-coil to link Nup85 of the two Y complexes and protrudes into the axial pore of the NPC. These previously uncharacterized structural features reveal insights into NPC assembly.

中文翻译:

非洲爪蟾核孔复合物胞质环的结构通过冷冻电子显微镜单颗粒分析。

核孔复合物(NPC)具有结构可塑性,并且仅在胞质环(CR)的局部分辨率高达15Å时才能表征。在这里,我们介绍了非洲爪蟾NPC CR的单粒子低温电子显微镜(cryo-EM)结构,平均分辨率为5.5-7.9Å,局部分辨率达到4.5Å。改进的分辨率允许在大多数CR组件中标识和放置辅助结构元素。每个CR亚基中的两个Y复合物彼此相互作用,并与侧翼亚基的Y复合物缔合,形成圆形支架。在每个CR亚基中,含有Nup358的区域包裹着两个Y复合物的茎,可能稳定了支架。Nup205连接两个Y配合物的短臂,并与相邻Y配合物的茎相连。包含Nup214的区域使用延伸的卷曲螺旋连接两个Y络合物的Nup85,并突出到NPC的轴向孔中。这些以前没有特征的结构特征揭示了对NPC组装的见识。
更新日期:2020-05-06
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