Objective

To characterize one linezolid- and methicillin-resistant Staphylococcus aureus (MRSA) isolate recovered from a nasal sample of a pig farmer patient.

Methods

The detection of linezolid resistance mechanisms was performed by PCR and sequencing. The antimicrobial resistance and virulence profile was investigated, and the molecular typing was performed by molecular techniques. The transference of cfr gene was assessed by conjugation experiments and its genetic environment was investigated by specific PCRs.

Results

The linezolid-resistant MRSA isolate was typed as t011-ST398/CC398-SCCmecV-agrI and carried the cfr gene. The isolate was multidrug-resistant but lacked the virulence genes studied. The cfr gene was co-located with the fexA gene on a Tn558 variant and was successfully transferred by conjugation.

Conclusion

We report the first description of LA-MRSA-CC398 carrying the cfr gene in Spain. This finding highlights the importance of surveillance programmes to determine the presence and spread of the cfr gene in the livestock and clinical settings.

中文翻译：

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在西班牙某养猪户中检测到 cfr 阳性 MRSA CC398 菌株

客观的

表征从猪农患者的鼻腔样本中提取的一种耐利奈唑胺和甲氧西林金黄色葡萄球菌(MRSA) 分离株。

方法

利奈唑胺耐药机制的检测通过 PCR 和测序进行。研究了抗菌素耐药性和毒力谱，并通过分子技术进行了分子分型。通过接合实验评估cfr基因的转移，并通过特异性 PCR 研究其遗传环境。

结果

耐利奈唑胺的 MRSA 分离株被分型为 t011-ST398/CC398-SCC mec V- agr I 并带有cfr基因。该分离株具有多重耐药性，但缺乏研究的毒力基因。的CFR基因共同定位与fexA上的TN基因558的变体，并通过缀合已成功传送。

结论

我们报告了西班牙对携带cfr基因的 LA-MRSA-CC398 的首次描述。这一发现强调了监测计划的重要性，以确定cfr基因在牲畜和临床环境中的存在和传播。