BACKGROUND Tick-borne phenuivirus (TBPVs) comprise human and animal viruses that can cause a variety of clinical syndromes ranging from self-limiting febrile illness to fatal haemorrhagic fevers. OBJECTIVE Detect Phlebovirus (Family Phenuiviridae) in ticks collected from domestic animals in Córdoba, Colombia. METHODS We collected 2365 ticks from domestic animals in three municipalities of the Department of Cordoba, Colombia in 2016. Ticks were identified and pooled by species for RNA extraction. A nested real-time PCR with specific primers for Phlebovirus and a specific probe for Heartland virus (HRTV) formerly a Phlebovirus, now a Banyangvirus were performed. Also, a conventional nested PCR, with the same specific primers was used to detect other Phleboviruses, with positive reactions indicated by an amplified cDNA fragment of approximately 244 bp determined by gel electrophoresis. These bands were gel-purified and sequenced by the Sanger method. RESULTS Using real-time RT-PCR, no positive results for HRTV were found. However, using conventional nested PCR 2.2% (5/229 pools) yielded a product of 244 bp. One positive sample was detected in a pool of Dermacentor nitens ticks collected from a horse, and the four remaining positive pools were from Rhipicephalus microplus collected from cattle. The five positive nucleotide sequences had identities of 93 to 96% compared to a section of the L-segment of Lihan Tick virus, a Phlebovirus originally detected in R. microplus ticks in China. The strongest identity (96-99%) was with Lihan Tick virus detected in R. microplus ticks from Brazil. CONCLUSIONS This is the first report of viral detection in ticks in Colombia. We detected a Colombian strain of Lihan Tick virus. We recommend expanding the sampling area and carrying out more eco-epidemiological studies related to epidemiological surveillance of viruses on ticks in Colombia.

中文翻译：

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Lihan Tick病毒（Phlebovirus，Phenuiviridae）在壁虱中的首次报告，哥伦比亚。

背景技术壁虱传播的肺病毒（TBPV）包括人和动物病毒，它们可引起多种临床综合征，包括自限性高热疾病到致命性出血热。目的检测在哥伦比亚科尔多瓦家畜中采集的tick中的猪瘟病毒（家族苯丙病毒科）。方法2016年，我们从哥伦比亚科尔多瓦省三个城市的家畜中采集了2365个tick虫。对Ti虫进行了鉴定，并按物种进行了汇集，以提取RNA。进行了嵌套实时PCR，其中包含针对细小病毒的特异引物和针对心地病毒（HRTV）的特异探针（以前称为细小病毒，现在为Banyang病毒）。同样，使用具有相同特异性引物的常规巢式PCR检测其他Phleboviruses，通过凝胶电泳确定的约244 bp的cDNA扩增片段可显示阳性反应。这些条带经过凝胶纯化，并通过Sanger方法测序。结果使用实时RT-PCR，未发现HRTV阳性。但是，使用常规的巢式PCR 2.2％（5/229池）可产生244 bp的产物。在从一匹马中收集到的Dermacentor nitens壁虱池中检测到一个阳性样本，其余四个阳性池来自从牛身上收集到的微小头蛇耳plus。与Lihan Tick病毒L段的一部分相比，这五个阳性核苷酸序列具有93％至96％的同一性。身份最强（96-99％）的病毒来自巴西的R. microplus s中检出的Lihan Tick病毒。结论这是哥伦比亚首次在壁虱中检测病毒的报道。我们检测到哥伦比亚的Lihan Tick病毒。我们建议扩大采样范围，并开展更多与哥伦比亚tick虫病毒流行病学监测有关的生态流行病学研究。