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Exploring of InDel in bovine PSAP gene and their association with growth traits in different development stages
Animal Biotechnology ( IF 3.7 ) Pub Date : 2020-05-05 , DOI: 10.1080/10495398.2020.1758122
Shuhui Wang 1 , Haidong Zhao 1 , Mingli Wu 1 , Xiaohua Yi 1 , Pingbo Chen 1 , Shirong Liu 1 , Yun Pan 1 , Qi Li 1 , Xiaoqin Tang 1 , Xiuzhu Sun 1, 2
Affiliation  

Abstract

PSAP (prosaposin) is widely expressed in different organs, and plays an important role in fat deposit. Insertion/Deletion (InDel) is a relatively simple and effective DNA marker. However, the association of molecular marker at different stages of animal development has not received enough attention, especially fat deposition related traits. Therefore, eight cattle breeds were used to explore novel InDels variants within bovine PSAP gene, and to evaluate their effects on growth traits in different development stages. Herein, two novel InDels (P5:NC037355.1g.27974439-27974440 ins AGTGTGGTTAATGTCAAC and P8:NC037355.1g.27980734-27980752 del GTCAAAAAATCAGGGGAAAC) within the bovine PSAP gene were found, and their association with growth traits in different development stages were analyzed. Interestingly, the dominant genotype was different in different development stages both in NY cattle and JX cattle for daily gain and body weight. PSAP Gene expression patterns were analyzed in this study, high expression in the middle stage of adipocytes differentiation suggests that it plays a certain role in fat development. It reveals that InDels could affect phenotype in different development stages, which depend on the expression pattern of the host gene and their function in different tissues. These findings could provide a new way for molecular marker studies in bovine breeding and genetics.



中文翻译:

牛PSAP基因InDel及其与不同发育阶段生长性状的相关性研究

摘要

PSAP(prosaposin)广泛表达于不同器官,在脂肪沉积中起重要作用。插入/缺失 (InDel) 是一种相对简单有效的 DNA 标记。然而,动物发育不同阶段分子标记的关联性并未得到足够的重视,尤其是脂肪沉积相关性状。因此,使用 8 个牛品种来探索牛PSAP基因中的新 InDels 变体,并评估它们对不同发育阶段生长性状的影响。在此,牛PSAP中的两个新的 InDels (P5:NC037355.1g.27974439-27974440 ins AGTGTGGTTAATGTCAAC 和 P8:NC037355.1g.27980734-27980752 del GTCAAAAAATCAGGGGAAAC)发现基因,并分析其与不同发育阶段生长性状的关系。有趣的是,NY牛和JX牛在不同发育阶段的显性基因型在日增重和体重方面是不同的。本研究分析了PSAP基因的表达模式,脂肪细胞分化中期的高表达表明它在脂肪发育中发挥了一定的作用。它揭示了InDels可以影响不同发育阶段的表型,这取决于宿主基因的表达模式及其在不同组织中的功能。这些发现可以为牛育种和遗传学中的分子标记研究提供新的途径。

更新日期:2020-05-05
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