Construction of a fast, easy and sensitive neurotransmitters-based sensor could provide a promising way for the diagnosis of neurological diseases, leading to the discovery of more effective treatment methods. The current work is directed to develop for the first time a flexible Surface-Enhanced Raman Spectroscopy (SERS) based neurotransmitters sensor by using the ultrasonic-assisted fabrication of a new set of epoxy resin (EPR) nanocomposites based on graphene nanosheets (GNS) using the casting technique. The perspicuous epoxy resin was reinforced by the variable loading of GNS giving the general formula GNS/EPR1–5. The designed products have been fabricated in situ while the perspicuous epoxy resin was formed. The expected nanocomposites have been fabricated using 3%, 5%, 10%, 15% and 20% GNS loading was applied for such fabrication process. The chemical, physical and morphological properties of the prepared nanocomposites were investigated by using Fourier transforms infrared spectroscopy, X-ray diffraction, Thermogravimetric analysis, Differential Thermal gravimetry, and field emission scanning electron microscopy methods. The GNS/EPR1–5 nanocomposites were decorated with a layer of gold nanoparticles (Au NPs/GNS/EPR) to create surface-enhanced Raman scattering hot points. The wettability of the Au NPs/GNS/EPR was investigated in comparison with the different nanocomposites and the bare epoxy. Au NPs/GNS/EPR was used as a SERS-active surface for detecting different concentrations of dopamine with a limit of detection of 3.3 µM. Our sensor showed the capability to detect low concentrations of dopamine either in a buffer system or in human serum as a real sample.

中文翻译：

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基于超声制造的金纳米颗粒/石墨烯/环氧树脂组成的纳米片，用于使用表面增强拉曼光谱的柔性多巴胺生物传感器。

快速，简便和灵敏的基于神经递质的传感器的构建可以为神经系统疾病的诊断提供有希望的方法，从而导致发现更有效的治疗方法。当前的工作是首次通过超声辅助制造基于石墨烯纳米片（GNS）的新型环氧树脂（EPR）纳米复合材料，开发出一种基于柔性表面增强拉曼光谱（SERS）的神经递质传感器。铸造技术。透明的环氧树脂通过GNS的可变载荷得到增强，给出的通式为GNS / EPR1-5。设计的产品是在形成透明环氧树脂的同时就地制造的。预期的纳米复合材料已经使用3％，5％，10％，15％和20％的GNS负载量进行了制备。通过傅立叶变换红外光谱，X射线衍射，热重分析，差示热重分析和场发射扫描电子显微镜方法研究了所制备纳米复合材料的化学，物理和形态学性质。用一层金纳米颗粒（Au NPs / GNS / EPR）装饰GNS / EPR1-5纳米复合材料，以产生表面增强的拉曼散射热点。与不同的纳米复合材料和裸露的环氧树脂相比，研究了Au NPs / GNS / EPR的润湿性。Au NPs / GNS / EPR用作SERS活性表面，用于检测不同浓度的多巴胺，检出限为3.3 µM。我们的传感器显示了在缓冲液系统或人血清中作为真实样品检测低浓度多巴胺的能力。通过傅立叶变换红外光谱，X射线衍射，热重分析，差示热重分析和场发射扫描电子显微镜方法研究了制备的纳米复合材料的物理和形态特性。用一层金纳米颗粒（Au NPs / GNS / EPR）装饰GNS / EPR1-5纳米复合材料，以产生表面增强的拉曼散射热点。与不同的纳米复合材料和裸露的环氧树脂相比，研究了Au NPs / GNS / EPR的润湿性。Au NPs / GNS / EPR用作SERS活性表面，用于检测不同浓度的多巴胺，检出限为3.3 µM。我们的传感器显示了在缓冲液系统或人血清中作为真实样品检测低浓度多巴胺的能力。通过傅立叶变换红外光谱，X射线衍射，热重分析，差示热重分析和场发射扫描电子显微镜方法研究了制备的纳米复合材料的物理和形态特性。用一层金纳米颗粒（Au NPs / GNS / EPR）装饰GNS / EPR1-5纳米复合材料，以产生表面增强的拉曼散射热点。与不同的纳米复合材料和裸露的环氧树脂相比，研究了Au NPs / GNS / EPR的润湿性。Au NPs / GNS / EPR用作SERS活性表面，用于检测不同浓度的多巴胺，检出限为3.3 µM。我们的传感器显示了在缓冲液系统或人血清中作为真实样品检测低浓度多巴胺的能力。