In all animal species, oxygen consumption is a key process that is partially impaired in a large number of pathological situations and thus provides informative details on the physiopathology of the disease. In this study, we describe a simple and affordable method to precisely measure oxygen consumption in living zebrafish larvae using a spectrofluorometer and the MitoXpress Xtra Oxygen Consumption Assay. In addition, we used zebrafish larvae treated with mitochondrial respiratory chain inhibitors, antimycin A or rotenone, to verify that our method enables precise and reliable measurements of oxygen consumption.

中文翻译：

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一种快速，简单且价格合理的技术来测量活斑马鱼胚胎中的氧气消耗。

在所有动物物种中，氧气消耗是一个关键过程，在许多病理情况下会部分受损，因此会提供有关该疾病的生理病理学的详细信息。在这项研究中，我们描述了一种简单且价格合理的方法，可以使用分光荧光计和MitoXpress Xtra耗氧量测定法精确测量活斑马鱼幼虫中的耗氧量。此外，我们使用经线粒体呼吸链抑制剂，抗霉素A或鱼藤酮处理的斑马鱼幼虫，来验证我们的方法能够精确可靠地测量耗氧量。