当前位置: X-MOL 学术Curr. Opin. Biotech. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
High-throughput screening for efficient microbial biotechnology.
Current Opinion in Biotechnology ( IF 7.7 ) Pub Date : 2020-04-14 , DOI: 10.1016/j.copbio.2020.02.019
Aditya Sarnaik 1 , Arren Liu 2 , David Nielsen 1 , Arul M Varman 1
Affiliation  

Microbial systems have been widely studied and exploited through genetic engineering to address industrial needs and societal challenges. However, owing to their complexity, singular approaches often do not yield desired or optimal results, pushing researchers to explore combinatorial strategies. With advances in synthetic biology, various methods can readily be employed to generate large and comprehensive libraries. To serve as tractable tools, however, this capability necessitates the development of high-throughput screening (HTS) techniques to identify the best performing strain and/or those carrying the desired trait. Owing to their miniaturization, time efficiency, potential for automation, and so on, HTS enables comprehensive exploration of diverse experimental landscapes. Herein, we review the recent and novel HTS approaches and applications in the realm of microbial biotechnology.

中文翻译:

高通量筛选,用于高效的微生物生物技术。

微生物系统已经通过基因工程进行了广泛的研究和开发,以解决工业需求和社会挑战。但是,由于其复杂性,单一方法通常无法产生理想或最佳结果,从而促使研究人员探索组合策略。随着合成生物学的进步,可以很容易地采用各种方法来生成大型而全面的文库。但是,要用作易于处理的工具,此功能需要开发高通量筛选(HTS)技术,以鉴定性能最佳的菌株和/或具有所需特征的菌株。由于其小型化,时间效率高,自动化潜力大等优点,HTS使得能够全面探索各种实验环境。在这里
更新日期:2020-04-14
down
wechat
bug