Dissecting neuronal structure and function in relation to behavior is an immense undertaking. Researchers require imaging tools to study neuronal activity and biochemical signaling in situ in order to study the roles of neuronal and biochemical activity in information processing. A large number of genetically encoded fluorescent biosensors have been reported in the literature over the past few years as there is a push to develop new technology in neuroscience. Here, we review the classes and characteristics of fluorescent biosensors and highlight some considerations that investigators should keep in mind when choosing their tool. In addition, we discuss recent advances in biosensor development.

中文翻译：

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用于神经元信号传导的荧光传感器。

解剖与行为有关的神经元结构和功能是一项艰巨的任务。为了研究神经元和生化活动在信息处理中的作用，研究人员需要成像工具来研究神经元活动和生化信号。在过去的几年中，随着对神经科学新技术的推动，文献中已经报道了许多遗传编码的荧光生物传感器。在这里，我们回顾了荧光生物传感器的类别和特性，并强调了研究人员在选择其工具时应牢记的一些注意事项。此外，我们讨论了生物传感器开发的最新进展。