Pseudomonads are well-known to thrive in diverse and complex nutritional habitats, and these capabilities make Pseudomonas species attractive as whole-cell biocatalysts. Industrial bioconversion processes often rely on complex uptake and synergistic metabolic systems due to the presence of varied carbon substrates in nutrient feedstocks. Isotope labeling experiments (ILEs) are emerging techniques used to elucidate cell metabolism following feeding on isotopically enriched substrates and are pivotal to the understanding of carbon partitioning during co-utilization. In this review, we highlight the applications of ILEs to decipher the metabolic networks in Pseudomonas species and evaluate their relevance in optimizing biocatalytic platforms.

中文翻译：

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假单胞菌物种中协同底物协同利用的13C同位素追踪进展及其对生物技术应用的意义。

假单胞菌在各种复杂的营养生境中壮成长，众所周知，这些功能使假单胞菌种具有作为全细胞生物催化剂的吸引力。由于营养原料中存在多种碳底物，工业生物转化过程通常依赖复杂的吸收和协同代谢系统。同位素标记实验（ILE）是新兴技术，用于阐明以同位素富集的底物为食后的细胞代谢，并且对于理解共同利用过程中碳的分配至关重要。在这篇综述中，我们重点介绍了ILE在假单胞菌物种中解密代谢网络并评估其在优化生物催化平台中的相关性的应用。