Abstract

In this article, we analyzed the structures of three acidic hetero-chain polysaccharides (CTP3-B, CTP3-C, and CTP3-D) fractionated from the herb Crepis crocea (Lam.) Babc. by a combination of ethanol precipitation, dialysis and gel permeation chromatography. Three polysaccharides were all highly branched polysaccharide. KK-Ay mice were chosen to determine the hypoglycemic effect of CTP3. The anti-diabetic activity of CTP3 was explored in detail from the aspects of body weight, daily dietary intake, blood glucose level and oral glucose tolerance test (OGTT). It was found that the body weight and daily food intake of the high dose group were significantly decreased compared with the diabetic control group. In addition, there was significant decrease in the levels of blood glucose in the middle and high dose group. These results suggest that CTP3 had a regulative role in blood glucose level. CTP3 may be useful in the treatment of type 2 diabetes mellitus.

摘要

在本文中，我们分析了从大黄花中提取的三种酸性杂链多糖（CTP3-B、CTP3-C 和 CTP3-D）的结构（Lam。）Babc。通过乙醇沉淀、透析和凝胶渗透色谱的组合。三种多糖均为高度支化的多糖。选择 KK-Ay 小鼠来确定 CTP3 的降血糖作用。从体重、每日膳食摄入量、血糖水平和口服葡萄糖耐量试验（OGTT）等方面详细探讨了CTP3的抗糖尿病活性。结果发现，与糖尿病对照组相比，高剂量组的体重和每日食物摄入量均显着降低。此外，中、高剂量组的血糖水平也有明显下降。这些结果表明CTP3对血糖水平具有调节作用。CTP3 可用于治疗 2 型糖尿病。