Background

It was found that regulatory T cells (Tregs) importantly affect the maintenance of the kidney graft. However, Tregs are a heterogeneous population with less to more suppressive activity. The aim of this study was to determine the effects of different subsets of Tregs, as well as their ratio to effector T cells (Teff), on kidney transplantation outcomes.

Methods

A total of 58 participants were enrolled in this study and divided into four groups: (i) first kidney transplant recipients (stable 1); (ii) second kidney transplant recipients (stable 2); (iii) transplant recipients with acute rejection (AR); and (iv) healthy control subjects. By using flow cytometer, the frequencies of CD4⁺ CD25⁺⁺ CD45RA⁻ Foxp3^hi activated Tregs (aTregs), CD4⁺ CD25⁺ CD45RA⁺ Foxp3^lo resting Tregs (rTregs), CD4⁺ CD25⁺ CD45RA⁻ Foxp3^lo non-suppressive T cells, CD4⁺ CD25⁺ Foxp3⁻ cells Teff, and total Tregs were analyzed in all subjects.

Results

The frequency of aTregs (as well as the ratio of aTregs/Tregs) was significantly lower in the AR patients than the other three groups. In contrast to AR patients, stables 1 and 2 had a higher aTreg/Treg ratio than those in the control group. Although patients with AR had a significantly lower total Tregs than the other three groups, the balance of total Tregs and Teff was similar between patients with and without AR.

Conclusion

Patients with AR had poorer immunoregulatory properties than those with normal graft functioning, as well as those in the control group. These reduced immunoregulatory properties in patients with AR could lead to graft rejection.

中文翻译：

---

肾移植患者中减少的CD4 + CD25 ++ CD45RA- Foxp3hi激活的调节性T细胞及其与急性排斥反应的关系。

背景

发现调节性T细胞（Tregs）重要地影响肾移植物的维持。但是，Tregs是异质性群体，具有较少或更多的抑制活性。这项研究的目的是确定不同种类的Treg的作用及其与效应T细胞（Teff）的比例对肾脏移植结果的影响。

方法

共有58名参与者参加了这项研究，分为四组：（i）第一批肾移植受者（稳定期1）；（ii）第二肾脏移植受者（稳定2）；（iii）急性排斥反应（AR）的移植受者；（iv）健康对照组。通过使用流式细胞仪，CD4 ⁺ CD25 ⁺⁺ CD45RA ^- Foxp3^高活化Tregs（aTregs），CD4 ⁺ CD25 ⁺ CD45RA ⁺ Foxp3 ^lo静止Tregs（rTregs），CD4 ⁺ CD25 ⁺ CD45RA ⁻ Foxp3 ^lo非抑制性T细胞的频率，CD4 ⁺ CD25 ⁺FOXP3 ^-细胞画眉草，和总调节性T细胞在所有科目进行了分析。

结果

AR患者中aTregs的频率（以及aTregs / Tregs的比率）显着低于其他三组。与AR患者相反，马s 1和马2 2的aTreg / Treg比值高于对照组。尽管AR患者的总Treg显着低于其他三组，但有和没有AR的患者总Treg和Teff的平衡相似。

结论

AR患者的免疫调节特性比正常移植功能正常的人群和对照组的免疫调节特性差。AR患者免疫调节特性的降低可能导致移植排斥。