This study aimed to characterize the effect of different running modes on serum irisin concentrations in rats. A total of 18, 10-week-old rats were divided into three groups; control group, 16° uphill running group (concentric exercise; CON) and, −16° downhill running group (eccentric exercise; ECC). The running group’s rats ran on the inclined treadmill at 16 m/min, for a total of 90 min. Blood was drawn from the rats, 48 h after running, after which the rats were anesthetized. The serum concentrations of irisin were measured using enzyme-linked immunosorbent assays. Vastus intermedius was collected for immunohistochemical analysis. After multiple comparisons, the ECC showed a significantly high serum irisin concentration (ECC: 28.42 ± 6.31 ng/ml, CON: 21.27 ± 3.03 ng/ml) and a larger irisin antibody reactive cross-sectional area in vastus intermedius compared to the CON (p < 0.05). This is the first study to reveal that single bout downhill running increases serum irisin concentrations in rats.

这项研究旨在表征不同运行模式对大鼠血清中鸢尾素浓度的影响。总共18只10周大的大鼠被分为三组。对照组，16°下坡跑步组（同心运动； CON）和-16°下坡跑步组（同心运动； ECC）。跑步组的大鼠以16 m / min的速度在倾斜跑步机上奔跑，总共进行90分钟。奔跑后48小时从大鼠中抽血，然后麻醉大鼠。使用酶联免疫吸附测定法测量虹膜素的血清浓度。收集中间阴静脉用于免疫组织化学分析。经过多次比较，ECC显示出很高的血清虹膜素浓度（ECC：28.42±6.31 ng / ml，CON：21.27±3。p  ＜0.05）。这是第一项揭示单次下坡跑步增加大鼠血清中鸢尾素浓度的研究。