Abstract

Ferulic acid is a kind of phenolic compound that can be found in various fruits and vegetables. This study aims to investigate the effect of ferulic acid on nephrotoxicity induced by gentamicin (GM). In this study, rats were separated into 4 groups such that each containing 8 randomly selected rats: Control group, Ferulic Acid (FA) group, Gentamicin (GM) group and Gentamicin + Ferulic acid (GM + FA) group. Blood samples were collected after 24 hours following the 8-day trial period, and kidneys were taken out for histopathological evaluation. Serum urea, creatinine, uric acid and LDH analyses were performed in autoanalyzer while Malondialdehyde (MDA), Advanced Oxidized Protein Products (AOPP), Glutathione (GSH), Superoxide dismutase (SOD), Catalase (CAT), Interleukin 6 (IL-6), Tumor Necrosis Factor-alpha (TNF-α) analyses were performed in ELISA, and kidney tissues were also examined histopathologically. Urea (p < .001), creatinine (p < .001), MDA (p < .01), AOPP (p < .001), IL-6 (p < .01) and TNF-α (p < .001) levels were found to be statistically and significantly lowered in GM + FA group when compared to GM group. As a result, ferulic acid has reduced the inflammation in nephrotoxicity induced by GM, causing decreased oxidative stress. In this study, anti-inflammatory features of ferulic acid have come to the forefront rather than the antioxidant features. It can be said that ferulic acid reduces nephrotoxic damage and has protective properties for kidneys.

摘要

阿魏酸是一种酚类化合物，存在于各种水果和蔬菜中。本研究旨在探讨阿魏酸对庆大霉素（GM）所致肾毒性的影响。在本研究中，将大鼠分成4组，每组随机选择8只大鼠：对照组、阿魏酸(FA)组、庆大霉素(GM)组和庆大霉素+阿魏酸(GM+FA)组。在为期 8 天的试验期后 24 小时后收集血样，并取出肾脏进行组织病理学评估。血清尿素、肌酐、尿酸和 LDH 分析在自动分析仪中进行，而丙二醛 (MDA)、高级氧化蛋白质产品 (AOPP)、谷胱甘肽 (GSH)、超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT)、白细胞介素 6 (IL-6 )，在 ELISA 中进行肿瘤坏死因子-α (TNF-α) 分析，和肾组织也进行了组织病理学检查。尿素（p  < .001)、肌酐 ( p  < .001)、MDA ( p  < .01)、AOPP ( p  < .001)、IL-6 ( p  < .01) 和 TNF-α ( p  < .001) 水平与 GM 组相比，GM + FA 组在统计学上显着降低。因此，阿魏酸减少了由 GM 引起的肾毒性炎症，从而降低了氧化应激。在这项研究中，阿魏酸的抗炎特性而不是抗氧化特性已经占据了首要位置。可以说，阿魏酸减少了肾毒性损伤，对肾脏有保护作用。