To investigate the effects of low-dose γ irradiation on apoptosis and development of the brain in zebrafish embryos, cumulative 15 mGy doses of γ rays from a 137Cs source were used to irradiate zebrafish embryos at 2 h post-fertilization (hpf) for 120 h. Apoptosis of the brain, brain morphological development, cell submicroscopic structure and mRNA expression were analyzed, respectively. Results indicate that after 15 mGy exposure, the apoptosis of zebrafish brain increased, vacuoles appeared in brain tissue, some organelles were damaged and vacuoles appeared locally in brain cells. The mRNA expression level of axin2 was significantly upregulated, and those of frizzled, β-catenin, camk2, TCF/ LEF and bcl9 were significantly downregulated in brain tissue. These genes are involved in the Wnt signaling pathway. The findings of this work suggest that low-dose radiation may influence the apoptosis and development of the brain in the zebrafish embryo by inhibiting the Wnt signaling pathway.

中文翻译：

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低剂量伽马射线辐射对斑马鱼胚胎脑细胞凋亡和发育的影响。

为了研究低剂量γ辐射对斑马鱼胚胎中细胞凋亡和大脑发育的影响，在受精后2 h（hpf）下使用累积15 mGy剂量的137Cs来源的γ射线照射斑马鱼胚胎120 h 。分别分析了脑的凋亡，脑形态发育，细胞亚显微结构和mRNA表达。结果表明，暴露于15 mGy后，斑马鱼脑细胞凋亡增加，在脑组织中出现液泡，一些细胞器被破坏，在脑细胞中局部出现液泡。脑组织中axin2的mRNA表达水平显着上调，而卷曲蛋白，β-catenin，camk2，TCF / LEF和bcl9的mRNA表达则显着下调。这些基因参与Wnt信号通路。