Long non-coding RNAs (lncRNAs) play a key role in the development and metastasis of cancer. However, the biological role and clinical significance of lncRNA DNAJC3-AS1 in the development of colon cancer is still unknown. In this study, the effects of DNAJC3-AS1 on cell proliferation, migration, and invasion were evaluated by MTT assay, wound-healing assay, and transwell assay, respectively. The relationship between DNAJC3-AS1, miR-214-3p and LIVIN was predicted by the online software and confirmed by dual-luciferase reporter assay. We found that the down-regulation of DNAJC3-AS1 inhibited the proliferation of colon cancer cells and induced growth arrest. Down-regulation of DNAJC3-AS1 also inhibited the migration, invasion, and epithelial-mesenchymal transition (EMT) of colon cancer cells. Moreover, miR-214-3p can bind to DNAJC3-AS1, and knockdown of DNAJC3-AS1 increased miR-214-3p expression in colon cancer cells. LIVIN was identified as a target of miR-214-3p. The up-regulation of miR-214-3p inhibited the protein expression of LIVIN and suppressed the activation of the NF-κB signaling pathway. Besides, down-regulation of DNAJC3-AS1 reduced cell viability, invasion, and EMT of colon cancer cells, while miR-214-3p inhibitor could reverse these effects. The expression of LIVIN and the activation of the NF-κB signaling pathway were suppressed by down-regulating DNAJC3-AS1, while these effects could be restored by miR-214-3p inhibitor. These findings suggested that DNAJC3-AS1 may promote colon cancer progression by regulating the miR-214-3p/LIVIN axis. DNAJC3-AS1 may serve as a new biomarker and therapeutic target for colon cancer, stimulating new research directions and treatment options.

中文翻译：

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lncRNA DNAJC3-AS1的下调通过调节miR-214-3p / LIVIN轴抑制结肠癌。

长的非编码RNA（lncRNA）在癌症的发展和转移中起关键作用。然而，lncRNA DNAJC3-AS1在结肠癌发展中的生物学作用和临床意义仍然未知。在这项研究中，分别通过MTT分析，伤口愈合分析和Transwell分析评估了DNAJC3-AS1对细胞增殖，迁移和侵袭的影响。DNAJC3-AS1，miR-214-3p和LIVIN之间的关系已通过在线软件预测，并通过双荧光素酶报告基因分析得以证实。我们发现DNAJC3-AS1的下调抑制结肠癌细胞的增殖并诱导生长停滞。DNAJC3-AS1的下调也抑制了结肠癌细胞的迁移，侵袭和上皮-间质转化（EMT）。而且，miR-214-3p可以与DNAJC3-AS1结合，DNAJC3-AS1的敲除和敲除可增加miR-214-3p在结肠癌细胞中的表达。LIVIN被确定为miR-214-3p的靶标。miR-214-3p的上调抑制了LIVIN的蛋白表达，并抑制了NF-κB信号通路的激活。此外，DNAJC3-AS1的下调降低了结肠癌细胞的细胞活力，侵袭和EMT，而miR-214-3p抑制剂可以逆转这些作用。下调DNAJC3-AS1可以抑制LIVIN的表达和NF-κB信号通路的激活，而miR-214-3p抑制剂可以恢复这些作用。这些发现表明，DNAJC3-AS1可能通过调节miR-214-3p / LIVIN轴促进结肠癌的进展。DNAJC3-AS1可作为结肠癌的新生物标志物和治疗靶标，