Coagulase-negative staphylococci, ubiquitous commensals of human skin, and mucous membranes represent important pathogens for immunocompromised patients and neonates. The increasing antibiotic resistance among Staphylococcus epidermidis is an emerging problem worldwide. In particular, the linezolid-resistant S. epidermidis (LRSE) strains are observed in Europe since 2014. The aim of our study was to genetically characterize 11 LRSE isolates, recovered mostly from blood in the University Children’s Hospital in Krakow, Poland, between 2015 and 2017. For identification of the isolates at the species level, we used 16S rRNA sequencing and RFLP of the saoC gene. Isolates were characterized phenotypically by determining their antimicrobial resistance patterns and using molecular methods such as PFGE, MLST, SCCmec typing, detection of the ica operon, and analysis of antimicrobial resistance determinants. All isolates were multidrug-resistant, including resistance to methicillin, and exhibited so-called PhLOPS_A phenotype. In PFGE, all isolates (excluding one from a catheter) represented identical patterns, were identified as ST2, and harbored the ica operon, responsible for biofilm formation. Linezolid resistance was associated with acquisition of A157R mutation in the ribosomal protein L3 and the presence of cfr gene. All isolates revealed new SCCmec cassette element composition. Recently, pediatric patients with serious staphylococcal infections are often treated with linezolid. The increasing linezolid resistance in bacterial strains becomes a real threat for patients, and monitoring such infections combined with surveillance and infection prevention programs is very important to decrease number of linezolid-resistant staphylococcal strains.

凝固酶阴性葡萄球菌，人体皮肤普遍存在的粘膜和粘膜是免疫受损患者和新生儿的重要病原体。表皮葡萄球菌之间日益增加的抗生素抗性是世界范围内新出现的问题。特别是，自2014年以来在欧洲观察到耐利奈唑酮的表皮葡萄球菌（LRSE）菌株。我们的研究目的是对11种LRSE分离株进行遗传学表征，这些分离株主要是从波兰克拉科夫的大学儿童医院于2015年之间从血液中回收的和2017年。为了在物种水平上鉴定菌株，我们使用了SaoC的16S rRNA测序和RFLP基因。通过确定其抗药性模式并使用分子方法（如PFGE，MLST，SCC mec分型，ica操纵子的检测以及抗药性决定因素的分析）对分离物进行表型表征。所有分离株均具有多重耐药性，包括对甲氧西林的耐药性，并表现出所谓的PhLOPS _A表型。在PFGE中，所有分离株（不包括导管中的分离株）都代表相同的模式，被鉴定为ST2，并带有负责生物膜形成的ica操纵子。利奈唑胺抗性与核糖体蛋白L3中A157R突变的获得和cfr基因的存在有关。所有分离株均发现新的SCCmec盒式磁带组成。最近，患有严重葡萄球菌感染的小儿患者经常用利奈唑胺治疗。细菌菌株中利奈唑胺耐药性的增加已成为患者的真正威胁，而监测此类感染并结合监测和预防感染计划对于减少耐利奈唑胺葡萄球菌菌株的数量非常重要。