During large-scale acute radiation exposure, rapidly distinguishing exposed individuals from nonexposed individuals is necessary. Identifying those exposed to high and potentially lethal radiation doses, and in need of immediate treatment, is especially important. To address this and find plasma biomarkers to assess ionizing radiation-induced mortality in the early stages, mice were administered a whole-body lethal dose of γ radiation, and radiation-induced damage was evaluated. Multiple blood biomarkers were screened using an antibody array, followed by validation using enzyme-linked immunoassay. The results revealed that irradiation (IR)-induced mortality in mice and caused body weight and blood platelet losses in deceased mice compared to surviving mice. The levels of certain proteins differed after IR between these 2 groups. Specific proteins in preirradiated mice were also found to potentiate radiosensitivity. Plasma levels of interleukin (IL)-22, urokinase, resistin, and IL-6 were associated with radiation-induced mortality in irradiated mice and may be useful as potential mortality predictors. Our results suggest that estimating the levels of certain plasma proteins is a promising alternative to conventional cytogenetic biodosimetry to accurately identify individuals exposed to high radiation doses and those at risk of death due to exposure. This strategy would facilitate the rapid triage of individuals requiring immediate and intensive medical treatment.

中文翻译：

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血浆蛋白作为小鼠全身照射后死亡率的生物标志物。

在大规模的急性辐射暴露中，必须迅速地区分暴露的个体和未暴露的个体。识别暴露于高剂量和潜在致命剂量并需要立即治疗的人尤其重要。为了解决这个问题并找到血浆生物标记物以评估电离辐射诱发的早期死亡，对小鼠给予了全身致死剂量的γ辐射，并评估了辐射诱发的损害。使用抗体阵列筛选多种血液生物标志物，然后使用酶联免疫测定法进行验证。结果表明，与存活小鼠相比，放射线（IR）诱导的小鼠死亡以及死者的体重和血小板损失。IR后，这两组之间某些蛋白质的水平有所不同。还发现预辐照小鼠中的特定蛋白质可增强放射敏感性。白细胞介素（IL）-22，尿激酶，抵抗素和IL-6的血浆水平与受辐射小鼠的辐射诱发死亡率相关，可能用作潜在的死亡率预测指标。我们的结果表明，估算某些血浆蛋白的水平是常规细胞遗传生物剂量测定法的有希望的替代方法，以准确地识别暴露于高辐射剂量的个体和因暴露而死亡的风险。这种策略将有助于迅速分流需要立即和加强治疗的个人。IL-6和IL-6与辐射致辐射的小鼠死亡率有关，可能可用作潜在的死亡率预测指标。我们的结果表明，估算某些血浆蛋白的水平是常规细胞遗传生物剂量测定法的有希望的替代方法，以准确地识别暴露于高辐射剂量的个体和因暴露而死亡的风险。这种策略将有助于迅速分流需要立即和加强治疗的个人。IL-6和IL-6与辐射致辐射的小鼠死亡率有关，可能可用作潜在的死亡率预测指标。我们的结果表明，估算某些血浆蛋白的水平是常规细胞遗传生物剂量测定法的有希望的替代方法，以准确地识别暴露于高辐射剂量的个体和因暴露而死亡的风险。该策略将有助于对需要立即和加强治疗的个人进行快速分类。