DNA damage repair and G2/M arrest are the key factors regulating the survival of cancer cells exposed to radiation. Recent studies have shown that long noncoding RNAs (lncRNAs) play important roles in a variety of biological processes, including DNA repair, cell cycle regulation, differentiation, and epigenetic regulation. However, the knowledge about the genome scale of lncRNAs and their potential biological functions in tumor cells exposed to radiation are still unclear. In this study, we used LncRNA + mRNA Human Gene Expression Microarray V4.0 to profile lncRNA and messenger RNA (mRNA) from HeLa, MCF-7, and A549 cells after irradiation with 4 Gy of γ-radiation. We identified 230, 227, and 274 differentially expressed lncRNAs and 150, 214, and 274 differentially expressed mRNAs in HeLa, MCF-7, and A549 cells, respectively, among which there are 14 common differentially expressed lncRNAs and 22 common differentially expressed mRNAs in all of the 3 cell lines. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis indicated that these differentially expressed mRNAs were mainly associated with cell cycle. Further, we also predicted the target genes and functions of these differentially expressed lncRNAs. Our study on lncRNAs has greatly expanded the field of gene research in the relationship of radiation, cell cycle, and DNA damage.

中文翻译：

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使用微阵列筛选辐射诱导的长非编码RNA。

DNA损伤修复和G2 / M阻滞是调节暴露于辐射的癌细胞存活的关键因素。最近的研究表明，长的非编码RNA（lncRNA）在多种生物学过程中起着重要作用，包括DNA修复，细胞周期调控，分化和表观遗传调控。然而，关于lncRNA的基因组规模及其在暴露于辐射的肿瘤细胞中的潜在生物学功能的知识仍不清楚。在这项研究中，我们使用LncRNA + mRNA人类基因表达微阵列V4.0对HeLa，MCF-7和A549细胞照射4 Gyγ射线后的lncRNA和信使RNA（mRNA）进行了分析。我们分别在HeLa，MCF-7和A549细胞中鉴定了230、227和274差异表达的lncRNA，以及150、214和274差异表达的mRNA，在这3种细胞系中，共有14个共同的差异表达lncRNA和22个共同的差异表达mRNA。基因本体论和京都基因与基因组百科全书通路分析表明，这些差异表达的mRNA主要与细胞周期有关。此外，我们还预测了这些差异表达的lncRNA的靶基因和功能。我们对lncRNA的研究极大地扩展了辐射，细胞周期和DNA损伤之间关系的基因研究领域。我们还预测了这些差异表达的lncRNA的靶基因和功能。我们对lncRNA的研究极大地扩展了辐射，细胞周期和DNA损伤之间关系的基因研究领域。我们还预测了这些差异表达的lncRNA的靶基因和功能。我们对lncRNA的研究极大地扩展了辐射，细胞周期和DNA损伤之间关系的基因研究领域。