This study aims to determine whether miR-1271-5p inhibits cell proliferation and enhances the radiosensitivity by targeting cyclin-dependent kinase 1 (CDK1) in hepatocellular carcinoma (HCC). Its expression levels in the HCC cell lines were significantly lower than those in normal human liver cell line. Bioinformatics analysis indicated CDK1 was a potential target of miR-1271-5p. Dual-Luciferase Reporter Assay confirmed that CDK1 is a direct target gene of miR-1271-5p. With overexpression of miR-1271-5p in SMMC-7721 and HuH-7 cells, cell proliferation was decreased, radiosensitivity was enhanced, cell cycle distribution was altered and the growth of transplanted tumours in nude mice was significantly reduced. miR-1271-5p overexpression enhanced radiosensitivity, which could be reduced by CDK1 overexpression. Overall, our findings suggested that miR-1271-5p inhibits cell proliferation and enhances the radiosensitivity of HCC cell lines by targeting CDK1.

中文翻译：

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MicroRNA-1271-5p通过靶向CDK1在肝细胞癌中抑制细胞增殖并增强放射敏感性。

这项研究旨在确定miR-1271-5p是否靶向肝细胞癌（HCC）中的细胞周期蛋白依赖性激酶1（CDK1），从而抑制细胞增殖并增强放射敏感性。HCC细胞系中其表达水平显着低于正常人肝细胞系中的表达水平。生物信息学分析表明CDK1是miR-1271-5p的潜在靶标。双重萤光素酶记者测定法证实CDK1是miR-1271-5p的直接靶基因。随着miR-1271-5p在SMMC-7721和HuH-7细胞中的过度表达，细胞增殖减少，放射敏感性增强，细胞周期分布改变，裸鼠移植瘤的生长明显减少。miR-1271-5p的过表达增强了放射敏感性，而CDK1的过表达可能会降低放射敏感性。总体，