INTRODUCTION AND PURPOSE Campylobacter jejuni and coli are zoonotic bacteria commonly associated With human diarrhea and usually transmit through consumption of meat and poultry contaminated products such as heart and liver. Cytolethal distending toxin (cdt) in Campylobacter spp. is among the significant virulence factors of these bacteria in the intestine. The purpose of this study was to determine the prevalence of Campylobacter spp. and presence of cdt genes among isolated bacteria. MATERIALS AND METHODS In this cross sectional study, 100 chicken livers were examined. Detection, bacterial enumeration and isolation of Campylobacter spp. was done using Campylobacter selective agar media containing Campylobacter growth supplement, gram staining, catalase and oxidase production, hippurate hydrolysis and PCR molecular technique. Also the presence of cdt genes were detected using PCR assay. RESULTS From 100 studied liver samples, 43 were contaminated with Campylobacter spp. Among them 31(72 %) samples had Campylobacter jejuni and 12 (28 %) had Campylobacter coli. All Campylobacter jejuni isolates contained cdtA ،cdtB and cdtC genes. However, all of these genes detected in 9 (75 %) of isolated coli. CONCLUSION The results of this study showed that great percentages of chicken livers in Tabriz were contaminated with Campylobacter.

中文翻译：

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从大不里士（Tabriz）的鸡肝中分离出弯曲杆菌种和细胞致死菌膨胀毒素的分子检测。

引言和目的空肠弯曲菌和大肠杆菌是人畜共患细菌，通常与人类腹泻有关，通常通过食用肉类和家禽污染的产品（例如心脏和肝脏）传播。弯曲杆菌属中的细胞致死性膨胀毒素（cdt）。这些细菌在肠道中的重要毒力因子之一。这项研究的目的是确定弯曲杆菌属的流行。和细菌分离物中cdt基因的存在。材料与方法在这项横断面研究中，检查了100只鸡肝。弯曲杆菌属的检测，细菌计数和分离。使用含有弯曲杆菌生长补充剂，革兰氏染色，过氧化氢酶和氧化酶生成，马尿酸盐水解和PCR分子技术的弯曲杆菌选择性琼脂培养基进行。还使用PCR测定法检测了cdt基因的存在。结果从100个研究的肝样本中，有43个被弯曲杆菌属菌污染。其中31个（72％）样本具有空肠弯曲菌，而12个样本（28％）具有大肠杆菌。所有空肠弯曲杆菌分离株均含有cdtA，cdtB和cdtC基因。但是，所有这些基因都在9个分离的大肠杆菌中检出（75％）。结论这项研究的结果表明，大不里士的很大一部分鸡肝都被弯曲杆菌污染。所有这些基因都在9个分离的大肠杆菌中检出（75％）。结论这项研究的结果表明，大不里士的很大一部分鸡肝都被弯曲杆菌污染。所有这些基因都在9个分离的大肠杆菌中检出（75％）。结论这项研究的结果表明，大不里士的很大一部分鸡肝都被弯曲杆菌污染。