Aim and Objective: Nipah virus (NiV) is a zoonotic virus of the paramyxovirus family that sporadically breaks out from livestock and spreads in humans through breathing resulting in an indication of encephalitis syndrome. In the current study, T cell epitopes with the NiV W protein antigens were predicted.

Materials and Methods: Modelling of unavailable 3D structure of W protein followed by docking studies of respective Human MHC - class I and MHC - class II alleles predicted was carried out for the highest binding rates. In the computational analysis, epitopes were assessed for immunogenicity, conservation, and toxicity analysis. T – cell-based vaccine development against NiV was screened for eight epitopes of Indian - Asian origin.

Results: Two epitopes, SPVIAEHYY and LVNDGLNII, have been screened and selected for further docking study based on toxicity and conservancy analyses. These epitopes showed a significant score of -1.19 kcal/mol and 0.15 kcal/mol with HLA- B*35:03 and HLA- DRB1 * 07:03, respectively by using allele - Class I and Class II from AutoDock. These two peptides predicted by the reverse vaccinology approach are likely to induce immune response mediated by T – cells.

Conclusion: Simulation using GROMACS has revealed that LVNDGLNII epitope forms a more stable complex with HLA molecule and will be useful in developing the epitope-based Nipah virus vaccine.

目的和目的：Nipah病毒（NiV）是副粘病毒家族的一种人畜共患病毒，它偶尔从牲畜中爆发并通过呼吸在人类中传播，从而导致脑炎综合征。在当前的研究中，预测了具有NiV W蛋白抗原的T细胞表位。

材料和方法：对W蛋白不可用的3D结构进行建模，然后对预测的各自人类MHC-I类和MHC-II类等位基因进行对接研究，以获得最高的结合率。在计算分析中，评估了抗原决定簇的免疫原性，保守性和毒性分析。针对印度—亚洲血统的8个抗原决定簇，筛选了针对NiV的T细胞疫苗研制。

结果：根据毒性和保守性分析，筛选并选择了两个表位，SPVIAEHYY和LVNDGLNII，用于进一步的对接研究。通过使用等位基因-AutoDock的I类和II类，这些表位在HLA- B * 35：03和HLA-DRB1 * 07:03时分别显示出-1.19 kcal / mol和0.15 kcal / mol的显着评分。通过反向疫苗学方法预测的这两种肽很可能诱导T细胞介导的免疫反应。

结论：使用GROMACS进行的模拟显示LVNDGLNII表位与HLA分子形成了更稳定的复合物，将有助于开发基于表位的Nipah病毒疫苗。