Liquid chromatography/electrospray ionization–tandem mass spectrometry (LC/ESI-MS/MS) enables the accurate and precise quantification of various analytes at very low concentrations, but it has room for improvement in analysis throughput. Multiplexing of samples in the same injection could be a promising procedure for enhancing the analysis throughput. This could be achieved by derivatization of the multiple samples with multiple isotopologous reagents. In this study, a sample-multiplexed LC/ESI-MS/MS assay using the 1-[(4-dimethylaminophenyl)carbonyl]piperazine (DAPPZ) isotopologues (²H₀-, ²H₃-, and ²H₆-forms) was developed and validated for the simultaneous determination of primary bile acids in three different plasma samples in a single run. The developed method had satisfactory intra- and inter-assay precisions (≤ 2.3 and ≤ 4.2%, respectively) and accuracy (99.0 – 100.3%), and could reduce the total LC/ESI-MS/MS run time by more than 60% for 42 samples compared to the conventional method. Thus, the derivatization with the DAPPZ isotopologues worked well for enhancing the throughput of the LC/ESI-MS/MS assay of the bile acids.

液相色谱/电喷雾电离串联质谱 (LC/ESI-MS/MS) 能够对极低浓度的各种分析物进行精确定量，但其分析通量仍有改进的空间。在同一注射中多重样品可能是提高分析通量的有前途的程序。这可以通过使用多种同位素试剂对多个样品进行衍生化来实现。在本研究中，使用 1-[(4-二甲基氨基苯基)羰基]哌嗪 (DAPPZ) 同位素体（ 2 H 0 -、2 H 3 - 和 2 H 6 - 形式^）进行_样品多重^LC / _ESI - ^MS / _MS测定）的开发和验证用于在一次运行中同时测定三种不同血浆样品中的初级胆汁酸。所开发的方法具有令人满意的测定内和测定间精密度（分别≤ 2.3 和 ≤ 4.2%）和准确度（99.0 – 100.3%），并且可以将总 LC/ESI-MS/MS 运行时间减少 60% 以上与传统方法相比，有 42 个样品。因此，用 DAPPZ 同位素体进行衍生化可以很好地提高胆汁酸的 LC/ESI-MS/MS 测定的通量。