Objectives

To test the effectiveness of an efficient therapeutic protocol for the total mouth antimicrobial photodynamic therapy (aPDT) mediated by 450 nm blue LED associated with curcumin in individuals with AIDS.

Methods

Patients were selected by exclusion criteria and randomly distributed in groups to test the effectiveness of antimicrobial aPDT with curcumin 0.75 mg/mL associated with the blue LED (67 mW/cm², 20.1 J/cm²). Before and after the treatments, samples were collected from the saliva being processed in duplicate in selective culture media. The colonies were counted and the results obtained in log10 CFU/mL were statistically tested (T-paired statistical test, 5%).

Results

The log10 CFU/mL of Streptococcus spp., Staphylococcus spp., and total count of microorganisms showed statistically significant (p = 0.023; p = 0.001 and p = 0.017, respectively) reduction after treatment in patients with aPDT.

Conclusion

aPDT was effective in reducing Streptococcusspp. in addition to reducing Staphylococcusspp., enterobacteria and the total count of microorganisms when considering the numbers of TCD4 and TCD8 lymphocytes. The aPDT in the studied protocol was able to control clinically important intraoral microorganisms for AIDS patients, both those with TCD4 lymphocytes above or below 25% of normal and those with TCD8 lymphocytes above 25% of normal.

中文翻译：

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蓝光和姜黄素介导的爱滋病患者的总口腔光动力疗法。

目标

为了测试有效的治疗方案对患有艾滋病的个体中与姜黄素相关的450 nm蓝色LED介导的全口抗菌光动力疗法（aPDT）的有效性。

方法

患者按排除标准选择并随机地分布在基姜黄素，以测试抗微生物APDT的有效性0.75与蓝色LED（67毫瓦/平方厘米相关毫克/毫升²，20.1焦耳/厘米²）。在处理之前和之后，从唾液中收集样品，并在选择性培养基中重复处理。对菌落计数并以log 10 CFU / mL获得的结果进行统计学检验（T配对统计学检验，5％）。

结果

aPDT患者治疗后，链球菌属，葡萄球菌属和微生物总数的log10 CFU / mL和微生物总数显示出统计学显着性降低（分别为p = 0.023，p = 0.001和p = 0.017）。

结论

aPDT可有效减少链球菌。考虑到TCD4和TCD8淋巴细胞的数量，除了减少葡萄球菌属，肠杆菌和微生物总数之外。研究方案中的aPDT能够控制AIDS患者的临床重要的口腔内微生物，包括TCD4淋巴细胞高于或低于正常值的25％以及TCD8淋巴细胞高于正常值的25％的患者。