Coating of titanium (Ti) implants with biocompatible polymers were performed to improve bone healing. In this study, pure Ti implants were coated via chitosan and alginate by spin coating method at 1000, 4000, and 8000 rpm. The coating layer was cross-linked by calcium chloride. Their chemical structures were analyzed by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) evaluations. The morphology of the created coating was observed by scanning electron microscopy (SEM), and the best uniformity was observed in the prepared coating at 8000 rpm (6093× g) spinal speed. The adhesion strength of the coating layer on the substrate was evaluated by the adhesion pull-off test. Also, the best adhesion strength was achieved at an 8000 rpm (6093× g) coating rate. Bioactivity of the chitosan-alginate coating on Ti sheets was evaluated by soaking the samples in a simulated body fluid (SBF) solution. The apatite formation on prepared Ti sheets was investigated by SEM, XRD, and energy dispersive X-ray spectroscopy (EDS). A higher mineralization appeared on coated samples compared with pure Ti. The antibacterial behavior of the implants was analyzed by bacterial counting against Escherichia coli. The presence of chitosan and alginate on the Ti sheets resulted in a better antibacterial effect. In-vitro experiments, with L929 fibroblast cells, confirmed the biocompatibility of the implants. Coating the Ti implants with chitosan and alginate improved biomineralization and biological behavior of the implant especially at the spinal speed of 8000 rpm (6093× g). These implants can support osteoblast cell adhesion and facilitate bone regeneration.

中文翻译：

---

钛涂层：通过旋涂在钛上引入抗菌和生物活性的壳聚糖-海藻酸盐薄膜。

用生物相容性聚合物对钛（Ti）植入物进行涂层以改善骨骼愈合。在这项研究中，纯钛植入物通过壳聚糖和藻酸盐通过旋涂方法以1000、4000和8000 rpm的速度涂覆。涂层通过氯化钙交联。通过傅立叶变换红外光谱（FTIR）和X射线衍射（XRD）评估分析了它们的化学结构。通过扫描电子显微镜（SEM）观察所形成的涂层的形态，并且在制备的涂层中以8000rpm（6093× g）的脊柱速度观察到最佳的均匀性。通过附着力剥离试验评价涂层在基材上的附着强度。同样，在8000 rpm（6093× g）涂布率。通过将样品浸泡在模拟体液（SBF）溶液中，评估了钛片上壳聚糖-海藻酸盐涂层的生物活性。通过SEM，XRD和能量色散X射线光谱法（EDS）研究了制备的Ti片上磷灰石的形成。与纯Ti相比，涂层样品的矿化度更高。通过对大肠杆菌的细菌计数分析了植入物的抗菌行为。钛片上壳聚糖和藻酸盐的存在导致更好的抗菌效果。体外用L929成纤维细胞进行的实验证实了植入物的生物相容性。用壳聚糖和藻酸盐涂覆Ti植入物可改善植入物的生物矿化和生物行为，尤其是在8000 rpm（6093× g）的脊柱速度下。这些植入物可以支持成骨细胞粘附并促进骨骼再生。